BAIT

UBL4A

DX254E, DXS254E, G6PD, GDX, GET5, MDY2, TMA24, UBL4, XX-FW89031B12.1
ubiquitin-like 4A
GO Process (2)
GO Function (2)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens
PREY

TUBA3E

tubulin, alpha 3e
GO Process (0)
GO Function (0)
GO Component (1)

Gene Ontology Cellular Component

Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

SGTA Recognizes a Noncanonical Ubiquitin-like Domain in the Bag6-Ubl4A-Trc35 Complex to Promote Endoplasmic Reticulum-Associated Degradation.

Xu Y, Cai M, Yang Y, Huang L, Ye Y

Elimination of aberrantly folded polypeptides from the endoplasmic reticulum (ER) by the ER-associated degradation (ERAD) system promotes cell survival under stress conditions. This quality control mechanism requires movement of misfolded proteins across the ER membrane for targeting to the cytosolic proteasome, a process facilitated by a "holdase" complex, consisting of Bag6 and the cofactors Ubl4A and Trc35. This multiprotein complex ... [more]

Cell Rep Dec. 27, 2012; 2(6);1633-44 [Pubmed: 23246001]

Throughput

  • High Throughput

Ontology Terms

  • cell line: hek-293 cell (BTO:0000007)

Additional Notes

  • Flag-tagged Ubl4a together with His-Bag6 is used to pull down interacting proteins which are identified by MS

Curated By

  • BioGRID