BAIT
UBL4A
DX254E, DXS254E, G6PD, GDX, GET5, MDY2, TMA24, UBL4, XX-FW89031B12.1
ubiquitin-like 4A
GO Process (2)
GO Function (2)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
SNRPD2
SMD2, SNRPD1, Sm-D2
small nuclear ribonucleoprotein D2 polypeptide 16.5kDa
GO Process (7)
GO Function (2)
GO Component (12)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- SMN-Sm protein complex [IDA]
- U1 snRNP [IDA]
- U12-type spliceosomal complex [IDA]
- U4 snRNP [IDA]
- catalytic step 2 spliceosome [IDA]
- cytosol [IDA, TAS]
- extracellular vesicular exosome [IDA]
- methylosome [IDA]
- nucleoplasm [TAS]
- pICln-Sm protein complex [IDA]
- small nuclear ribonucleoprotein complex [TAS]
- spliceosomal complex [TAS]
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
SGTA Recognizes a Noncanonical Ubiquitin-like Domain in the Bag6-Ubl4A-Trc35 Complex to Promote Endoplasmic Reticulum-Associated Degradation.
Elimination of aberrantly folded polypeptides from the endoplasmic reticulum (ER) by the ER-associated degradation (ERAD) system promotes cell survival under stress conditions. This quality control mechanism requires movement of misfolded proteins across the ER membrane for targeting to the cytosolic proteasome, a process facilitated by a "holdase" complex, consisting of Bag6 and the cofactors Ubl4A and Trc35. This multiprotein complex ... [more]
Cell Rep Dec. 27, 2012; 2(6);1633-44 [Pubmed: 23246001]
Throughput
- High Throughput
Ontology Terms
- cell line: hek-293 cell (BTO:0000007)
Additional Notes
- Flag-tagged Ubl4a together with His-Bag6 is used to pull down interacting proteins which are identified by MS
Curated By
- BioGRID