BAIT
UBL4A
DX254E, DXS254E, G6PD, GDX, GET5, MDY2, TMA24, UBL4, XX-FW89031B12.1
ubiquitin-like 4A
GO Process (2)
GO Function (2)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
TMED10
P24(DELTA), S31I125, S31III125, TMP21, Tmp-21-I, p23
transmembrane emp24-like trafficking protein 10 (yeast)
GO Process (10)
GO Function (2)
GO Component (13)
Gene Ontology Biological Process
- COPI coating of Golgi vesicle [TAS]
- COPI-coated vesicle budding [IDA]
- COPII vesicle coating [TAS]
- ER to Golgi vesicle-mediated transport [TAS]
- cargo loading into vesicle [TAS]
- intracellular protein transport [IDA]
- regulated secretory pathway [ISS]
- regulation of beta-amyloid formation [IMP]
- retrograde vesicle-mediated transport, Golgi to ER [ISS]
- vesicle targeting, to, from or within Golgi [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- COPI-coated vesicle [ISS]
- Golgi apparatus [IDA]
- Golgi membrane [IDA, TAS]
- cis-Golgi network [ISS]
- endoplasmic reticulum [IDA]
- endoplasmic reticulum-Golgi intermediate compartment [IDA]
- extracellular vesicular exosome [IDA]
- gamma-secretase complex [IDA]
- integral component of membrane [ISS]
- plasma membrane [ISS]
- secretory granule membrane [ISS]
- trans-Golgi network transport vesicle [ISS]
- zymogen granule membrane [ISS]
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
SGTA Recognizes a Noncanonical Ubiquitin-like Domain in the Bag6-Ubl4A-Trc35 Complex to Promote Endoplasmic Reticulum-Associated Degradation.
Elimination of aberrantly folded polypeptides from the endoplasmic reticulum (ER) by the ER-associated degradation (ERAD) system promotes cell survival under stress conditions. This quality control mechanism requires movement of misfolded proteins across the ER membrane for targeting to the cytosolic proteasome, a process facilitated by a "holdase" complex, consisting of Bag6 and the cofactors Ubl4A and Trc35. This multiprotein complex ... [more]
Cell Rep Dec. 27, 2012; 2(6);1633-44 [Pubmed: 23246001]
Throughput
- High Throughput
Ontology Terms
- cell line: hek-293 cell (BTO:0000007)
Additional Notes
- Flag-tagged Ubl4a together with His-Bag6 is used to pull down interacting proteins which are identified by MS
Curated By
- BioGRID