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BAIT

UBL4A

DX254E, DXS254E, G6PD, GDX, GET5, MDY2, TMA24, UBL4, XX-FW89031B12.1

ubiquitin-like 4A

GO Process (2)

GO Function (2)

GO Component (2)

Gene Ontology Biological Process

cellular protein modification process [TAS]

tail-anchored membrane protein insertion into ER membrane [IMP]

Gene Ontology Molecular Function

protein binding [IPI]
small conjugating protein transferase activity [TAS]

Gene Ontology Cellular Component

BAT3 complex [IDA]

CRISPR Database VEGA OMIM HGNC Alliance of Genome Resources Entrez Gene RefSeq UniprotKB Ensembl HPRD

Homo sapiens

PREY

RPS19

DBA, DBA1, S19

ribosomal protein S19

GO Process (26)

GO Function (6)

GO Component (8)

Gene Ontology Biological Process

RNA metabolic process [TAS]

SRP-dependent cotranslational protein targeting to membrane [TAS]

cellular protein metabolic process [TAS]

erythrocyte differentiation [IMP]

gene expression [TAS]

mRNA metabolic process [TAS]

maturation of SSU-rRNA [IMP]

maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [IMP]

monocyte chemotaxis [IDA]

negative regulation of respiratory burst involved in inflammatory response [IDA]

nuclear-transcribed mRNA catabolic process, nonsense-mediated decay [TAS]

nucleolus organization [IMP]

positive regulation of cellular component movement [TAS]

positive regulation of respiratory burst involved in inflammatory response [IDA]

protein tetramerization [IDA]

rRNA processing [IMP]

response to extracellular stimulus [TAS]

ribosomal small subunit assembly [IMP]

ribosomal small subunit biogenesis [IMP]

translation [IC, TAS]

translational elongation [TAS]

translational initiation [TAS]

translational termination [TAS]

viral life cycle [TAS]

viral process [TAS]

viral transcription [TAS]

Gene Ontology Molecular Function

fibroblast growth factor binding [IPI]
poly(A) RNA binding [IDA]
protein binding [IPI]
protein homodimerization activity [IDA]
protein kinase binding [IPI]
structural constituent of ribosome [IDA]

Gene Ontology Cellular Component

cytoplasm [IDA]

cytosol [IDA, TAS]

cytosolic small ribosomal subunit [IDA]

extracellular vesicular exosome [IDA]

focal adhesion [IDA]

nucleolus [IDA]

CRISPR Database OMIM HGNC Alliance of Genome Resources Entrez Gene RefSeq UniprotKB HPRD

Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

SGTA Recognizes a Noncanonical Ubiquitin-like Domain in the Bag6-Ubl4A-Trc35 Complex to Promote Endoplasmic Reticulum-Associated Degradation.

Xu Y, Cai M, Yang Y, Huang L, Ye Y

Elimination of aberrantly folded polypeptides from the endoplasmic reticulum (ER) by the ER-associated degradation (ERAD) system promotes cell survival under stress conditions. This quality control mechanism requires movement of misfolded proteins across the ER membrane for targeting to the cytosolic proteasome, a process facilitated by a "holdase" complex, consisting of Bag6 and the cofactors Ubl4A and Trc35. This multiprotein complex ... [more]

Cell Rep Dec. 27, 2012; 2(6);1633-44 [Pubmed: 23246001]

Throughput

High Throughput

Ontology Terms

cell line: hek-293 cell (BTO:0000007)

Additional Notes

Flag-tagged Ubl4a together with His-Bag6 is used to pull down interacting proteins which are identified by MS

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
RPS19 UBL4A	Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.	Havugimana PC (2012)	High	0.883	BioGRID	748184

Curated By

BioGRID