BAIT
ITGA4
CD49D, IA4
integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor)
GO Process (14)
GO Function (2)
GO Component (6)
Gene Ontology Biological Process
- B cell differentiation [IC]
- blood coagulation [TAS]
- cell-matrix adhesion [IMP]
- cell-matrix adhesion involved in ameboidal cell migration [IMP]
- endodermal cell differentiation [IEP]
- extracellular matrix organization [TAS]
- heterotypic cell-cell adhesion [IMP]
- leukocyte cell-cell adhesion [IDA]
- leukocyte migration [TAS]
- leukocyte tethering or rolling [IMP]
- negative regulation of protein homodimerization activity [IDA]
- receptor clustering [IMP]
- regulation of immune response [TAS]
- substrate adhesion-dependent cell spreading [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
HSPA8
HEL-33, HEL-S-72p, HSC54, HSC70, HSC71, HSP71, HSP73, HSPA10, LAP-1, LAP1, NIP71
heat shock 70kDa protein 8
GO Process (14)
GO Function (11)
GO Component (13)
Gene Ontology Biological Process
- ATP catabolic process [IDA]
- RNA metabolic process [TAS]
- axon guidance [TAS]
- gene expression [TAS]
- mRNA metabolic process [TAS]
- membrane organization [TAS]
- negative regulation of fibril organization [IDA]
- negative regulation of transcription, DNA-templated [IDA]
- neurotransmitter secretion [TAS]
- post-Golgi vesicle-mediated transport [TAS]
- protein folding [NAS]
- protein refolding [IDA]
- response to unfolded protein [NAS]
- synaptic transmission [TAS]
Gene Ontology Molecular Function- ATP binding [IDA]
- ATPase activity [IDA]
- ATPase activity, coupled [NAS]
- G-protein coupled receptor binding [IPI]
- MHC class II protein complex binding [IDA]
- enzyme binding [IPI]
- heat shock protein binding [IPI]
- poly(A) RNA binding [IDA]
- protein binding [IPI]
- ubiquitin protein ligase binding [IPI]
- unfolded protein binding [IDA]
- ATP binding [IDA]
- ATPase activity [IDA]
- ATPase activity, coupled [NAS]
- G-protein coupled receptor binding [IPI]
- MHC class II protein complex binding [IDA]
- enzyme binding [IPI]
- heat shock protein binding [IPI]
- poly(A) RNA binding [IDA]
- protein binding [IPI]
- ubiquitin protein ligase binding [IPI]
- unfolded protein binding [IDA]
Gene Ontology Cellular Component
- Prp19 complex [IDA]
- blood microparticle [IDA]
- clathrin-sculpted gamma-aminobutyric acid transport vesicle membrane [TAS]
- cytosol [IDA, TAS]
- extracellular space [IDA]
- extracellular vesicular exosome [IDA]
- focal adhesion [IDA]
- intracellular [NAS]
- membrane [IDA]
- nucleus [IDA]
- plasma membrane [TAS]
- ribonucleoprotein complex [IDA]
- ubiquitin ligase complex [IDA]
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Proteomic analysis of α4β1 integrin adhesion complexes reveals α-subunit-dependent protein recruitment.
Integrin adhesion receptors mediate cell-cell and cell-extracellular matrix interactions, which control cell morphology and migration, differentiation, and tissue integrity. Integrins recruit multimolecular adhesion complexes to their cytoplasmic domains, which provide structural and mechanosensitive signaling connections between the extracellular and intracellular milieux. The different functions of specific integrin heterodimers, such as α4β1 and α5β1, have been attributed to distinct signal transduction ... [more]
Proteomics Jul. 01, 2012; 12(13);2107-14 [Pubmed: 22623428]
Throughput
- Low Throughput
Ontology Terms
- k-562 cell (BTO:0000664)
- chronic myeloid leukemia (DOID:8552)
Additional Notes
- exogenous expression of bait
Curated By
- BioGRID