BAIT
ITGA4
CD49D, IA4
integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor)
GO Process (14)
GO Function (2)
GO Component (6)
Gene Ontology Biological Process
- B cell differentiation [IC]
- blood coagulation [TAS]
- cell-matrix adhesion [IMP]
- cell-matrix adhesion involved in ameboidal cell migration [IMP]
- endodermal cell differentiation [IEP]
- extracellular matrix organization [TAS]
- heterotypic cell-cell adhesion [IMP]
- leukocyte cell-cell adhesion [IDA]
- leukocyte migration [TAS]
- leukocyte tethering or rolling [IMP]
- negative regulation of protein homodimerization activity [IDA]
- receptor clustering [IMP]
- regulation of immune response [TAS]
- substrate adhesion-dependent cell spreading [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
PICALM
CALM, CLTH, LAP
phosphatidylinositol binding clathrin assembly protein
GO Process (22)
GO Function (5)
GO Component (12)
Gene Ontology Biological Process
- cargo loading into vesicle [IMP]
- cell proliferation [IMP]
- clathrin coat assembly [IMP]
- clathrin-mediated endocytosis [IMP]
- endosomal transport [IMP]
- iron ion homeostasis [IMP]
- iron ion import into cell [IMP]
- negative regulation of gene expression [IMP]
- negative regulation of metalloendopeptidase activity involved in amyloid precursor protein catabolic process [ISS]
- negative regulation of receptor-mediated endocytosis [IDA]
- positive regulation of aspartic-type endopeptidase activity involved in amyloid precursor protein catabolic process [ISS]
- positive regulation of beta-amyloid formation [IMP]
- positive regulation of neuron death [IMP]
- positive regulation of transcription, DNA-templated [IDA]
- protein complex assembly [TAS]
- receptor internalization [IMP]
- receptor-mediated endocytosis [IDA, ISS]
- regulation of aspartic-type endopeptidase activity involved in amyloid precursor protein catabolic process [IMP]
- regulation of endocytosis [IMP]
- regulation of protein localization [IDA]
- synaptic vesicle maturation [ISS]
- vesicle-mediated transport [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- AP-2 adaptor complex [IDA]
- clathrin coat of coated pit [IDA]
- coated pit [IDA, ISS]
- intracellular membrane-bounded organelle [IDA]
- membrane [IDA]
- neurofibrillary tangle [IMP]
- neuronal cell body [IDA]
- nucleus [IDA]
- perinuclear region of cytoplasm [ISS]
- postsynaptic membrane [ISS]
- presynaptic membrane [ISS]
- vesicle [ISS]
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Proteomic analysis of α4β1 integrin adhesion complexes reveals α-subunit-dependent protein recruitment.
Integrin adhesion receptors mediate cell-cell and cell-extracellular matrix interactions, which control cell morphology and migration, differentiation, and tissue integrity. Integrins recruit multimolecular adhesion complexes to their cytoplasmic domains, which provide structural and mechanosensitive signaling connections between the extracellular and intracellular milieux. The different functions of specific integrin heterodimers, such as α4β1 and α5β1, have been attributed to distinct signal transduction ... [more]
Proteomics Jul. 01, 2012; 12(13);2107-14 [Pubmed: 22623428]
Throughput
- Low Throughput
Ontology Terms
- cell line: k-562 cell (BTO:0000664)
- disease: chronic myeloid leukemia (DOID:8552)
Additional Notes
- exogenous expression of bait
Curated By
- BioGRID