BAIT

ITGA4

CD49D, IA4
integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor)
Homo sapiens
PREY

ESYT1

FAM62A, MBC2
extended synaptotagmin-like protein 1
GO Process (0)
GO Function (2)
GO Component (3)

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Proteomic analysis of α4β1 integrin adhesion complexes reveals α-subunit-dependent protein recruitment.

Byron A, Humphries JD, Craig SE, Knight D, Humphries MJ

Integrin adhesion receptors mediate cell-cell and cell-extracellular matrix interactions, which control cell morphology and migration, differentiation, and tissue integrity. Integrins recruit multimolecular adhesion complexes to their cytoplasmic domains, which provide structural and mechanosensitive signaling connections between the extracellular and intracellular milieux. The different functions of specific integrin heterodimers, such as α4β1 and α5β1, have been attributed to distinct signal transduction ... [more]

Proteomics Jul. 01, 2012; 12(13);2107-14 [Pubmed: 22623428]

Throughput

  • Low Throughput

Ontology Terms

  • cell line: k-562 cell (BTO:0000664)
  • disease: chronic myeloid leukemia (DOID:8552)

Additional Notes

  • exogenous expression of bait

Curated By

  • BioGRID