BAIT
ITGA4
CD49D, IA4
integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor)
GO Process (14)
GO Function (2)
GO Component (6)
Gene Ontology Biological Process
- B cell differentiation [IC]
- blood coagulation [TAS]
- cell-matrix adhesion [IMP]
- cell-matrix adhesion involved in ameboidal cell migration [IMP]
- endodermal cell differentiation [IEP]
- extracellular matrix organization [TAS]
- heterotypic cell-cell adhesion [IMP]
- leukocyte cell-cell adhesion [IDA]
- leukocyte migration [TAS]
- leukocyte tethering or rolling [IMP]
- negative regulation of protein homodimerization activity [IDA]
- receptor clustering [IMP]
- regulation of immune response [TAS]
- substrate adhesion-dependent cell spreading [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
IQGAP1
HUMORFA01, SAR1, p195
IQ motif containing GTPase activating protein 1
GO Process (13)
GO Function (9)
GO Component (17)
Gene Ontology Biological Process
- cellular response to calcium ion [IDA]
- cellular response to epidermal growth factor stimulus [IMP]
- energy reserve metabolic process [TAS]
- epidermal growth factor receptor signaling pathway [IMP]
- glomerular visceral epithelial cell development [ISS]
- negative regulation of catalytic activity [TAS]
- neuron projection extension [IMP]
- positive regulation of GTPase activity [TAS]
- positive regulation of protein kinase activity [IMP]
- positive regulation of protein serine/threonine kinase activity [IDA]
- regulation of insulin secretion [TAS]
- signal transduction [TAS]
- small molecule metabolic process [TAS]
Gene Ontology Molecular Function- GTPase activator activity [TAS]
- GTPase inhibitor activity [TAS]
- calcium ion binding [TAS]
- calmodulin binding [IPI]
- phosphatidylinositol-3,4,5-trisphosphate binding [IDA]
- protein binding [IPI]
- protein kinase binding [IPI]
- protein phosphatase binding [IPI]
- protein serine/threonine kinase activator activity [IDA]
- GTPase activator activity [TAS]
- GTPase inhibitor activity [TAS]
- calcium ion binding [TAS]
- calmodulin binding [IPI]
- phosphatidylinositol-3,4,5-trisphosphate binding [IDA]
- protein binding [IPI]
- protein kinase binding [IPI]
- protein phosphatase binding [IPI]
- protein serine/threonine kinase activator activity [IDA]
Gene Ontology Cellular Component
- actin cytoskeleton [ISS]
- actin filament [TAS]
- axon [ISS]
- cell junction [IDA]
- cytoplasm [IDA]
- cytoplasmic ribonucleoprotein granule [IDA]
- extracellular vesicular exosome [IDA]
- extrinsic component of cytoplasmic side of plasma membrane [IDA]
- focal adhesion [IDA]
- growth cone [ISS]
- microtubule [IDA]
- microtubule cytoskeleton [ISS]
- midbody [IDA]
- neuron projection [ISS]
- nucleoplasm [IDA]
- plasma membrane [IDA, TAS]
- slit diaphragm [ISS]
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Proteomic analysis of α4β1 integrin adhesion complexes reveals α-subunit-dependent protein recruitment.
Integrin adhesion receptors mediate cell-cell and cell-extracellular matrix interactions, which control cell morphology and migration, differentiation, and tissue integrity. Integrins recruit multimolecular adhesion complexes to their cytoplasmic domains, which provide structural and mechanosensitive signaling connections between the extracellular and intracellular milieux. The different functions of specific integrin heterodimers, such as α4β1 and α5β1, have been attributed to distinct signal transduction ... [more]
Proteomics Jul. 01, 2012; 12(13);2107-14 [Pubmed: 22623428]
Throughput
- High Throughput
Ontology Terms
- cell line: k-562 cell (BTO:0000664)
- disease: chronic myeloid leukemia (DOID:8552)
Additional Notes
- exogenous expression of bait
Curated By
- BioGRID