An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Fine mapping of the alpha-actinin binding site within cysteine-rich protein.

Harper BD, Beckerle MC, Pomies P

The cysteine-rich proteins (CRPs) are a family of highly conserved LIM (an acronym derived from the three gene products lin-11, isl-1 and mec-3) domain proteins that have been implicated in muscle differentiation. All CRP family members characterized so far have been shown to interact with the filamentous actin cross-linker alpha-actinin. The region of CRP required for this interaction has previously ... [more]

Biochem. J. Aug. 15, 2000; 350(0);269-74 [Pubmed: 10926853]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

ACTN1

Gene Ontology Biological Process

Gene Ontology Molecular Function

double-stranded RNA binding [IDA]integrin binding [IDA]ion channel binding [IPI]protein binding [IPI]vinculin binding [IPI]

Gene Ontology Cellular Component

CSRP1