BAIT

RSBN1

C230004D03Rik, E330004N01, Rosbin, Rsbn, Rsbp, mKIAA3002, AC162922.1
rosbin, round spermatid basic protein 1
GO Process (0)
GO Function (1)
GO Component (1)

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Mus musculus
PREY

PRKACA

PKCD, Pkaca
protein kinase, cAMP dependent, catalytic, alpha
GO Process (20)
GO Function (10)
GO Component (16)
Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Rosbin: a novel homeobox-like protein gene expressed exclusively in round spermatids.

Takahashi T, Tanaka H, Iguchi N, Kitamura K, Chen Y, Maekawa M, Nishimura H, Ohta H, Miyagawa Y, Matsumiya K, Okuyama A, Nishimune Y

Mammalian spermiogenesis is a complex process occurring in a highly coordinated fashion within the seminiferous tubules. To elucidate the molecular mechanisms controlling haploid germ cell differentiation, we have isolated haploid germ cell- specific cDNA clones from a subtracted cDNA library of mouse testis. One of these cDNAs, Rosbin, is 3.2 kilobases (kb) long and has an open reading frame of ... [more]

Biol. Reprod. May. 01, 2004; 70(5);1485-92 [Pubmed: 14724137]

Throughput

  • Low Throughput

Curated By

  • BioGRID