BAIT
RAG1
Rag-1, RP23-325I3.3
recombination activating gene 1
GO Process (16)
GO Function (9)
GO Component (1)
Gene Ontology Biological Process
- B cell differentiation [IDA, IMP, ISO]
- DNA recombination [TAS]
- T cell differentiation in thymus [IMP, ISO]
- T cell homeostasis [IGI]
- V(D)J recombination [IGI, IMP, ISO]
- adaptive immune response [IMP]
- death [IMP]
- histone monoubiquitination [IDA, ISO]
- negative regulation of T cell apoptotic process [IGI]
- negative regulation of cysteine-type endopeptidase activity involved in apoptotic process [IMP]
- negative regulation of thymocyte apoptotic process [IMP]
- nucleic acid phosphodiester bond hydrolysis [IMP, ISO]
- pre-B cell allelic exclusion [IMP, ISO]
- protein autoubiquitination [IDA, ISO]
- regulation of T cell differentiation [IGI]
- thymus development [IGI]
Gene Ontology Molecular Function- endonuclease activity [IMP, ISO]
- histone binding [IDA, ISO]
- metal ion binding [IMP, ISO]
- protein binding [IPI]
- protein homodimerization activity [IDA, ISO]
- sequence-specific DNA binding [IDA, IMP, ISO]
- ubiquitin protein ligase activity [IDA, ISO]
- ubiquitin-protein transferase activity [IDA, ISO]
- zinc ion binding [IDA, ISO]
- endonuclease activity [IMP, ISO]
- histone binding [IDA, ISO]
- metal ion binding [IMP, ISO]
- protein binding [IPI]
- protein homodimerization activity [IDA, ISO]
- sequence-specific DNA binding [IDA, IMP, ISO]
- ubiquitin protein ligase activity [IDA, ISO]
- ubiquitin-protein transferase activity [IDA, ISO]
- zinc ion binding [IDA, ISO]
Mus musculus
PREY
KPNA2
2410044B12Rik, IPOA1, PTAC58, Rch1, RP23-170A23.5
karyopherin (importin) alpha 2
GO Process (2)
GO Function (5)
GO Component (5)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- cell [IDA]
- cytoplasm [ISO]
- intracellular [IDA]
- membrane [ISO]
- nucleoplasm [ISO]
Mus musculus
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
A WW-like module in the RAG1 N-terminal domain contributes to previously unidentified protein-protein interactions.
More than one-third of the RAG1 protein can be truncated from the N-terminus with only subtle effects on the products of V(D)J recombination in vitro or in a mouse. What, then, is the function of the N-terminal domain? We believe it to be regulatory. We determined, several years ago, that an included RING motif could function as an ubiquitin E3 ... [more]
Nucleic Acids Res. Jun. 01, 2009; 37(10);3301-9 [Pubmed: 19324890]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| RAG1 KPNA2 | Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex. | High | 0.697 | BioGRID | 2681787 |
Curated By
- BioGRID