PREY

ATP6V0D2

1620401A02Rik, AI324824, V-ATPase, RP23-326I8.1
ATPase, H+ transporting, lysosomal V0 subunit D2
Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

a4, a unique kidney-specific isoform of mouse vacuolar H+-ATPase subunit a.

Oka T, Murata Y, Namba M, Yoshimizu T, Toyomura T, Yamamoto A, Sun-Wada GH, Hamasaki N, Wada Y, Futai M

The vacuolar-type H+ -ATPase (V-ATPase) translocates protons across membranes. Here, we have identified a mouse cDNA coding for a fourth isoform (a4) of the membrane sector subunit a of V-ATPase. This isoform was specifically expressed in kidney, but not in the heart, brain, spleen, lung, liver, muscle, or testis. Immunoprecipitation experiments, together with sequence similarities for other isoforms (a1, a2, ... [more]

J. Biol. Chem. Oct. 26, 2001; 276(43);40050-4 [Pubmed: 11498539]

Throughput

  • Low Throughput

Curated By

  • BioGRID