An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Ykt6 forms a SNARE complex with syntaxin 5, GS28, and Bet1 and participates in a late stage in endoplasmic reticulum-Golgi transport.

Zhang T, Hong W

The yeast SNARE Ykt6p has been implicated in several trafficking steps, including vesicular transport from the endoplasmic reticulum (ER) to the Golgi, intra-Golgi transport, and homotypic vacuole fusion. The functional role of its mammalian homologue (Ykt6) has not been established. Using antibodies specific for mammalian Ykt6, it is revealed that it is found mainly in Golgi-enriched membranes. Three SNAREs, syntaxin ... [more]

J. Biol. Chem. Jul. 20, 2001; 276(29);27480-7 [Pubmed: 11323436]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

YKT6

Gene Ontology Biological Process

Gene Ontology Molecular Function

SNAP receptor activity [IBA, ISO]SNARE binding [IBA]protein-cysteine S-palmitoyltransferase activity [ISO, ISS]

Gene Ontology Cellular Component

GOSR1