CYTH2
Gene Ontology Biological Process
Gene Ontology Molecular Function
ARF6
Gene Ontology Biological Process
- actin cytoskeleton organization [TAS]
- cortical actin cytoskeleton organization [ISO]
- establishment of epithelial cell polarity [ISO]
- hepatocyte apoptotic process [ISO]
- liver development [ISO]
- myeloid cell apoptotic process [ISO]
- negative regulation of dendrite development [IMP]
- positive regulation of actin filament polymerization [ISO]
- positive regulation of establishment of protein localization to plasma membrane [ISO]
- protein localization to cell surface [ISO]
- protein localization to endosome [ISO, ISS]
- regulation of Rac protein signal transduction [ISO]
- regulation of dendritic spine development [IMP, ISO]
- regulation of filopodium assembly [ISO]
- ruffle organization [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- cell cortex [ISO]
- cytoplasm [ISO]
- early endosome [IDA]
- endocytic vesicle [ISO, ISS]
- endosome [ISO]
- extracellular vesicular exosome [ISO]
- filopodium membrane [ISO]
- focal adhesion [ISO]
- membrane [ISO]
- myelin sheath [ISO]
- plasma membrane [IDA, ISO]
- recycling endosome [IDA]
- recycling endosome membrane [ISO, ISS]
- ruffle [IDA, ISO]
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Arf nucleotide binding site opener [ARNO] promotes sequential activation of Arf6, Cdc42 and Rac1 and insulin secretion in INS 832/13 β-cells and rat islets.
Glucose-stimulated insulin secretion [GSIS] involves interplay between small G-proteins and their regulatory factors. Herein, we tested the hypothesis that Arf nucleotide binding site opener [ARNO], a guanine nucleotide-exchange factor [GEF] for the small G-protein Arf6, mediates the functional activation of Arf6, and that ARNO/Arf6 signaling axis, in turn, controls the activation of Cdc42 and Rac1, which have been implicated in ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID