An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Human mediator subunit MED26 functions as a docking site for transcription elongation factors.

Takahashi H, Parmely TJ, Sato S, Tomomori-Sato C, Banks CA, Kong SE, Szutorisz H, Swanson SK, Martin-Brown S, Washburn MP, Florens L, Seidel CW, Lin C, Smith ER, Shilatifard A, Conaway RC, Conaway JW

Promoter-proximal pausing by initiated RNA polymerase II (Pol II) and regulated release of paused polymerase into productive elongation has emerged as a major mechanism of transcription activation. Reactivation of paused Pol II correlates with recruitment of super-elongation complexes (SECs) containing ELL/EAF family members, P-TEFb, and other proteins, but the mechanism of their recruitment is an unanswered question. Here, we present ... [more]

Cell Jul. 08, 2011; 146(1);92-104 [Pubmed: 21729782]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
CCNC MED26	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Marcon E (2014)	High	39.6602	BioGRID	2943306

Curated By

BioGRID

Interaction Summary

MED26

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA polymerase II transcription cofactor activity [IDA]protein binding [IPI]transcription coactivator activity [TAS]

Gene Ontology Cellular Component

CCNC