BAIT

CFTR

ABC35, ABCC7, CF, CFTR/MRP, MRP7, TNR-CFTR, dJ760C5.1, tcag7.78

cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7)

GO Process (9)

GO Function (9)

GO Component (10)

Gene Ontology Biological Process

cellular response to cAMP [ISS]

chloride transmembrane transport [IDA, ISS, NAS, TAS]

intracellular pH elevation [ISS]

membrane hyperpolarization [ISS]

positive regulation of voltage-gated chloride channel activity [IDA]

respiratory gaseous exchange [TAS]

sperm capacitation [ISS]

transmembrane transport [TAS]

transport [TAS]

Gene Ontology Molecular Function

ATP-binding and phosphorylation-dependent chloride channel activity [TAS]
PDZ domain binding [IDA]
bicarbonate transmembrane transporter activity [ISS]
channel-conductance-controlling ATPase activity [NAS]
chloride channel activity [IDA]
chloride channel inhibitor activity [IDA]
chloride transmembrane transporter activity [ISS]
enzyme binding [IPI]
protein binding [IPI]

Gene Ontology Cellular Component

apical plasma membrane [IDA]

basolateral plasma membrane [NAS]

cell surface [IDA]

cytoplasm [IDA]

early endosome [IDA]

endoplasmic reticulum Sec complex [IDA]

extracellular vesicular exosome [IDA]

plasma membrane [IDA, TAS]

protein complex [IDA]

recycling endosome [IDA]

CRISPR Database HGNC Alliance of Genome Resources VEGA OMIM Entrez Gene RefSeq UniprotKB Ensembl HPRD

Homo sapiens

PREY

ATP2A2

ATP2B, DAR, DD, SERCA2

ATPase, Ca++ transporting, cardiac muscle, slow twitch 2

GO Process (17)

GO Function (7)

GO Component (10)

Gene Ontology Biological Process

blood coagulation [TAS]

calcium ion import into sarcoplasmic reticulum [IC, ISS]

calcium ion transmembrane transport [IDA]

calcium ion transport from cytosol to endoplasmic reticulum [IDA]

cell adhesion [TAS]

cellular calcium ion homeostasis [IDA]

endoplasmic reticulum calcium ion homeostasis [IDA]

epidermis development [TAS]

ion transmembrane transport [TAS]

positive regulation of endoplasmic reticulum calcium ion concentration [IDA]

positive regulation of heart rate [TAS]

regulation of cardiac muscle cell action potential involved in regulation of contraction [ISS]

regulation of cardiac muscle cell membrane potential [IC, ISS, TAS]

regulation of cardiac muscle contraction by calcium ion signaling [IDA]

relaxation of cardiac muscle [IDA]

sarcoplasmic reticulum calcium ion transport [TAS]

transmembrane transport [TAS]

Gene Ontology Molecular Function

S100 protein binding [IPI]
calcium ion binding [IDA]
calcium-transporting ATPase activity [IDA]
calcium-transporting ATPase activity involved in regulation of cardiac muscle cell membrane potential [IC, ISS, TAS]
enzyme binding [IPI]
protein C-terminus binding [IPI]
protein binding [IPI]

CRISPR Database HGNC Alliance of Genome Resources OMIM VEGA Entrez Gene RefSeq UniprotKB Ensembl HPRD

Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Hsp90 cochaperone Aha1 downregulation rescues misfolding of CFTR in cystic fibrosis.

Wang X, Venable J, LaPointe P, Hutt DM, Koulov AV, Coppinger J, Gurkan C, Kellner W, Matteson J, Plutner H, Riordan JR, Kelly JW, Yates JR, Balch WE

The pathways that distinguish transport of folded and misfolded cargo through the exocytic (secretory) pathway of eukaryotic cells remain unknown. Using proteomics to assess global cystic fibrosis (CF) transmembrane conductance regulator (CFTR) protein interactions (the CFTR interactome), we show that Hsp90 cochaperones modulate Hsp90-dependent stability of CFTR protein folding in the endoplasmic reticulum (ER). Cell-surface rescue of the most common ... [more]

Cell Nov. 17, 2006; 127(4);803-15 [Pubmed: 17110338]

Throughput

High Throughput

Ontology Terms

ht-29 cell (BTO:0000182) [colon adenocarcinoma (DOID:234)]
colorectal adenocarcinoma cell line (BTO:0001914) [colorectal cancer (DOID:9256)]

Additional Notes

T84 cell

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
CFTR ATP2A2	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Matos AM (2019)	Low	-	BioGRID	-
CFTR ATP2A2	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Pankow S (2015)	High	-	BioGRID	2447884

Curated By

BioGRID