BAIT
VCP
valosin-containing protein
GO Process (19)
GO Function (13)
GO Component (15)
Gene Ontology Biological Process
- ATP catabolic process [IDA, IMP]
- ER to Golgi vesicle-mediated transport [IMP]
- ER-associated ubiquitin-dependent protein catabolic process [ISO, ISS]
- activation of cysteine-type endopeptidase activity involved in apoptotic process [ISO]
- aggresome assembly [ISO]
- cellular response to DNA damage stimulus [ISO, ISS]
- double-strand break repair [ISO, ISS]
- positive regulation of Lys63-specific deubiquitinase activity [ISO]
- positive regulation of proteasomal ubiquitin-dependent protein catabolic process [IMP, ISO]
- positive regulation of protein K63-linked deubiquitination [ISO]
- positive regulation of protein catabolic process [ISO]
- positive regulation of protein complex assembly [ISO]
- protein N-linked glycosylation via asparagine [ISO, ISS]
- protein hexamerization [IDA]
- protein homooligomerization [IDA]
- protein ubiquitination [ISO, ISS]
- retrograde protein transport, ER to cytosol [ISO]
- translesion synthesis [ISO, ISS]
- ubiquitin-dependent protein catabolic process [ISO]
Gene Ontology Molecular Function- ADP binding [IMP]
- ATP binding [IDA, IMP]
- ATPase activity [IDA, IMP]
- deubiquitinase activator activity [ISO]
- identical protein binding [IDA]
- poly(A) RNA binding [ISO]
- polyubiquitin binding [ISO]
- protein binding [IPI]
- protein complex binding [IPI]
- protein domain specific binding [ISO]
- protein phosphatase binding [ISO]
- receptor binding [IDA]
- ubiquitin-specific protease binding [ISO]
- ADP binding [IMP]
- ATP binding [IDA, IMP]
- ATPase activity [IDA, IMP]
- deubiquitinase activator activity [ISO]
- identical protein binding [IDA]
- poly(A) RNA binding [ISO]
- polyubiquitin binding [ISO]
- protein binding [IPI]
- protein complex binding [IPI]
- protein domain specific binding [ISO]
- protein phosphatase binding [ISO]
- receptor binding [IDA]
- ubiquitin-specific protease binding [ISO]
Gene Ontology Cellular Component
- Hrd1p ubiquitin ligase complex [ISO]
- cytoplasm [ISO]
- cytosol [IDA, ISO]
- endoplasmic reticulum [IDA, ISO]
- endoplasmic reticulum membrane [ISO]
- extracellular vesicular exosome [ISO]
- intracellular membrane-bounded organelle [IDA]
- lipid particle [ISO]
- myelin sheath [ISO]
- nucleoplasm [ISO]
- nucleus [ISO]
- perinuclear region of cytoplasm [ISO]
- proteasome complex [ISO]
- protein complex [ISO]
- site of double-strand break [ISO, ISS]
Rattus norvegicus
PREY
UBE4A
Ab2-232
ubiquitination factor E4A
GO Process (1)
GO Function (2)
GO Component (1)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Rattus norvegicus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
A complex of mammalian ufd1 and npl4 links the AAA-ATPase, p97, to ubiquitin and nuclear transport pathways.
The AAA-ATPase, p97/Cdc48p, has been implicated in many different pathways ranging from membrane fusion to ubiquitin-dependent protein degradation. Binding of the p47 complex directs p97 to act in the post-mitotic fusion of Golgi membranes. We now describe another binding complex comprising mammalian Ufd1 and Npl4. Yeast Ufd1p is required for ubiquitin-dependent protein degradation whereas yeast Npl4p has been implicated in ... [more]
EMBO J. May. 15, 2000; 19(10);2181-92 [Pubmed: 10811609]
Throughput
- Low Throughput
Curated By
- BioGRID