BAIT

POP2

CAF1, CCR4-NOT core DEDD family RNase subunit POP2, L000001465, YNR052C
RNase of the DEDD superfamily; subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation
Saccharomyces cerevisiae (S288c)
PREY

AAC3

ANC3, ADP/ATP carrier protein AAC3, L000000005, YBR085W
Mitochondrial inner membrane ADP/ATP translocator; exchanges cytosolic ADP for mitochondrially synthesized ATP; expressed under anaerobic conditions; similar to Aac1p; has roles in maintenance of viability and in respiration; AAC3 has a paralog, PET9, that arose from the whole genome duplication
GO Process (3)
GO Function (1)
GO Component (3)
Saccharomyces cerevisiae (S288c)

Synthetic Growth Defect

A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

Publication

Large-scale investigation of oxygen response mutants in Saccharomyces cerevisiae.

Samanfar B, Omidi K, Hooshyar M, Laliberte B, Alamgir M, Seal AJ, Ahmed-Muhsin E, Viteri DF, Said K, Chalabian F, Golshani A, Wainer G, Burnside D, Shostak K, Bugno M, Willmore WG, Smith ML, Golshani A

A genome-wide screen of a yeast non-essential gene-deletion library was used to identify sick phenotypes due to oxygen deprivation. The screen provided a manageable list of 384 potentially novel as well as known oxygen responding (anoxia-survival) genes. The gene-deletion mutants were further assayed for sensitivity to ferrozine and cobalt to obtain a subset of 34 oxygen-responsive candidate genes including the ... [more]

Mol Biosyst Mar. 07, 2013; 0(0); [Pubmed: 23467670]

Throughput

  • High Throughput|Low Throughput

Ontology Terms

  • phenotype: vegetative growth (APO:0000106)
  • phenotype: colony size (APO:0000063)

Additional Notes

  • High Throughput: SGA analysis
  • Low Throughput: random spore analysis to confirm HTP result

Curated By

  • BioGRID