BAIT
UBC
ubiquitin C
GO Process (2)
GO Function (2)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Rattus norvegicus
PREY
HMGCR
3H3M
3-hydroxy-3-methylglutaryl-CoA reductase
GO Process (22)
GO Function (6)
GO Component (3)
Gene Ontology Biological Process
- aging [IEP]
- cholesterol biosynthetic process [IDA, ISO]
- farnesyl diphosphate biosynthetic process, mevalonate pathway [TAS]
- isoprenoid biosynthetic process [IDA]
- myoblast differentiation [IEP]
- negative regulation of MAP kinase activity [ISO]
- negative regulation of apoptotic process [IMP]
- negative regulation of insulin secretion involved in cellular response to glucose stimulus [IMP]
- negative regulation of striated muscle cell apoptotic process [IMP]
- negative regulation of vasodilation [IMP]
- negative regulation of wound healing [IMP]
- positive regulation of ERK1 and ERK2 cascade [IMP]
- positive regulation of cardiac muscle cell apoptotic process [IMP]
- positive regulation of cell proliferation [IMP]
- positive regulation of skeletal muscle tissue development [IMP]
- positive regulation of smooth muscle cell proliferation [IMP]
- positive regulation of stress-activated MAPK cascade [IMP]
- protein tetramerization [ISO]
- response to ethanol [IEP]
- response to nutrient [IEP]
- ubiquinone metabolic process [IMP]
- visual learning [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Rattus norvegicus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Synaptic Protein Ubiquitination in Rat Brain Revealed by Antibody-based Ubiquitome Analysis.
Protein ubiquitination is an essential post-translational modification regulating neurodevelopment, synaptic plasticity, learning, and memory, and its dysregulation contributes to the pathogenesis of neurological diseases. Here we report a systematic analysis of ubiquitinated proteome (ubiquitome) in rat brain using a newly developed monoclonal antibody that recognizes the diglycine tag on lysine residues in trypsinized peptides (K-GG peptides). Initial antibody specificity analysis ... [more]
J. Proteome Res. Aug. 15, 2012; 0(0); [Pubmed: 22871113]
Throughput
- Low Throughput
Curated By
- BioGRID