BAIT
UBC
ubiquitin C
GO Process (2)
GO Function (2)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Rattus norvegicus
PREY
RPS3
ribosomal protein S3
GO Process (9)
GO Function (10)
GO Component (12)
Gene Ontology Biological Process
- DNA catabolic process, endonucleolytic [IBA, ISO]
- DNA repair [IBA]
- cellular response to DNA damage stimulus [ISO]
- cytoplasmic translation [IBA]
- negative regulation of DNA repair [ISO]
- positive regulation of DNA N-glycosylase activity [ISO]
- positive regulation of NF-kappaB transcription factor activity [ISO]
- positive regulation of apoptotic signaling pathway [ISO]
- translation [IC]
Gene Ontology Molecular Function- DNA-(apurinic or apyrimidinic site) lyase activity [ISO]
- NF-kappaB binding [ISO]
- damaged DNA binding [IBA, ISO]
- enzyme binding [ISO]
- mRNA binding [ISO]
- oxidized purine nucleobase lesion DNA N-glycosylase activity [IBA]
- poly(A) RNA binding [ISO]
- protein kinase A binding [ISO]
- protein kinase binding [ISO]
- structural constituent of ribosome [IBA, IC, ISO]
- DNA-(apurinic or apyrimidinic site) lyase activity [ISO]
- NF-kappaB binding [ISO]
- damaged DNA binding [IBA, ISO]
- enzyme binding [ISO]
- mRNA binding [ISO]
- oxidized purine nucleobase lesion DNA N-glycosylase activity [IBA]
- poly(A) RNA binding [ISO]
- protein kinase A binding [ISO]
- protein kinase binding [ISO]
- structural constituent of ribosome [IBA, IC, ISO]
Gene Ontology Cellular Component
Rattus norvegicus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Synaptic Protein Ubiquitination in Rat Brain Revealed by Antibody-based Ubiquitome Analysis.
Protein ubiquitination is an essential post-translational modification regulating neurodevelopment, synaptic plasticity, learning, and memory, and its dysregulation contributes to the pathogenesis of neurological diseases. Here we report a systematic analysis of ubiquitinated proteome (ubiquitome) in rat brain using a newly developed monoclonal antibody that recognizes the diglycine tag on lysine residues in trypsinized peptides (K-GG peptides). Initial antibody specificity analysis ... [more]
J. Proteome Res. Aug. 15, 2012; 0(0); [Pubmed: 22871113]
Throughput
- Low Throughput
Curated By
- BioGRID