BAIT
UBC
ubiquitin C
GO Process (2)
GO Function (2)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Rattus norvegicus
PREY
HTR2A
5-HT2A, 5Ht-2
5-hydroxytryptamine (serotonin) receptor 2A, G protein-coupled
GO Process (33)
GO Function (7)
GO Component (8)
Gene Ontology Biological Process
- G-protein coupled receptor signaling pathway [IDA]
- activation of phospholipase C activity [ISO]
- aging [IEP]
- artery smooth muscle contraction [IMP]
- behavioral response to cocaine [IDA]
- cell death [IMP]
- cellular calcium ion homeostasis [ISO]
- detection of mechanical stimulus involved in sensory perception of pain [IMP]
- detection of temperature stimulus involved in sensory perception of pain [IMP]
- memory [IMP]
- negative regulation of potassium ion transport [IMP]
- negative regulation of synaptic transmission, glutamatergic [IMP]
- phosphatidylinositol 3-kinase signaling [ISO]
- phospholipase C-activating serotonin receptor signaling pathway [IDA]
- positive regulation of ERK1 and ERK2 cascade [ISO]
- positive regulation of MAP kinase activity [IMP]
- positive regulation of cell proliferation [IMP]
- positive regulation of cytosolic calcium ion concentration [IMP]
- positive regulation of fat cell differentiation [ISO]
- positive regulation of glycolytic process [ISO]
- positive regulation of kinase activity [ISO]
- positive regulation of peptidyl-tyrosine phosphorylation [ISO]
- positive regulation of phosphatidylinositol biosynthetic process [ISO]
- positive regulation of vasoconstriction [IMP]
- protein localization to cytoskeleton [ISO]
- regulation of dopamine secretion [IMP]
- release of sequestered calcium ion into cytosol [ISO]
- response to drug [IMP, ISO]
- sensory perception of pain [IMP]
- serotonin receptor signaling pathway [ISO]
- sleep [IMP]
- temperature homeostasis [IMP]
- urinary bladder smooth muscle contraction [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Rattus norvegicus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Synaptic Protein Ubiquitination in Rat Brain Revealed by Antibody-based Ubiquitome Analysis.
Protein ubiquitination is an essential post-translational modification regulating neurodevelopment, synaptic plasticity, learning, and memory, and its dysregulation contributes to the pathogenesis of neurological diseases. Here we report a systematic analysis of ubiquitinated proteome (ubiquitome) in rat brain using a newly developed monoclonal antibody that recognizes the diglycine tag on lysine residues in trypsinized peptides (K-GG peptides). Initial antibody specificity analysis ... [more]
J. Proteome Res. Aug. 15, 2012; 0(0); [Pubmed: 22871113]
Throughput
- Low Throughput
Curated By
- BioGRID