BAIT
UBC
ubiquitin C
GO Process (2)
GO Function (2)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Rattus norvegicus
PREY
ARFGEF1
BIG1, RGD1560793
ADP-ribosylation factor guanine nucleotide-exchange factor 1(brefeldin A-inhibited)
GO Process (9)
GO Function (6)
GO Component (9)
Gene Ontology Biological Process
- Golgi organization [ISO, ISS]
- endomembrane system organization [ISO, ISS]
- negative regulation of Rho GTPase activity [ISO, ISS]
- negative regulation of actin filament polymerization [ISO, ISS]
- positive regulation of GTPase activity [IBA, ISO]
- positive regulation of protein glycosylation in Golgi [ISO, ISS]
- positive regulation of wound healing [ISO, ISS]
- regulation of establishment of cell polarity [ISO, ISS]
- vesicle-mediated transport [IBA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Rattus norvegicus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Synaptic Protein Ubiquitination in Rat Brain Revealed by Antibody-based Ubiquitome Analysis.
Protein ubiquitination is an essential post-translational modification regulating neurodevelopment, synaptic plasticity, learning, and memory, and its dysregulation contributes to the pathogenesis of neurological diseases. Here we report a systematic analysis of ubiquitinated proteome (ubiquitome) in rat brain using a newly developed monoclonal antibody that recognizes the diglycine tag on lysine residues in trypsinized peptides (K-GG peptides). Initial antibody specificity analysis ... [more]
J. Proteome Res. Aug. 15, 2012; 0(0); [Pubmed: 22871113]
Throughput
- Low Throughput
Curated By
- BioGRID