BAIT
UBC
ubiquitin C
GO Process (2)
GO Function (2)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Rattus norvegicus
PREY
ANXA5
Anx5, LC5
annexin A5
GO Process (6)
GO Function (4)
GO Component (14)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- Z disc [IDA]
- cell projection [IDA]
- cytoplasm [IDA]
- endothelial microparticle [ISO]
- external side of plasma membrane [ISO]
- extracellular space [IDA]
- extracellular vesicular exosome [ISO]
- focal adhesion [ISO]
- intercalated disc [IDA]
- intracellular [ISO]
- membrane [ISO]
- nucleus [IDA]
- plasma membrane [IDA]
- sarcolemma [IDA]
Rattus norvegicus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Synaptic Protein Ubiquitination in Rat Brain Revealed by Antibody-based Ubiquitome Analysis.
Protein ubiquitination is an essential post-translational modification regulating neurodevelopment, synaptic plasticity, learning, and memory, and its dysregulation contributes to the pathogenesis of neurological diseases. Here we report a systematic analysis of ubiquitinated proteome (ubiquitome) in rat brain using a newly developed monoclonal antibody that recognizes the diglycine tag on lysine residues in trypsinized peptides (K-GG peptides). Initial antibody specificity analysis ... [more]
J. Proteome Res. Aug. 15, 2012; 0(0); [Pubmed: 22871113]
Throughput
- Low Throughput
Curated By
- BioGRID