BAIT
UBC
ubiquitin C
GO Process (2)
GO Function (2)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Rattus norvegicus
PREY
APP
Abeta
amyloid beta (A4) precursor protein
GO Process (37)
GO Function (10)
GO Component (31)
Gene Ontology Biological Process
- adult locomotory behavior [ISO, ISS]
- axon cargo transport [ISO, ISS]
- axon midline choice point recognition [ISO, ISS]
- axonogenesis [ISO, ISS]
- cellular copper ion homeostasis [ISO, ISS]
- cholesterol metabolic process [ISO]
- collateral sprouting in absence of injury [ISO, ISS]
- dendrite development [ISO, ISS]
- endocytosis [ISO, ISS]
- extracellular matrix organization [ISO, ISS]
- forebrain development [ISO]
- ionotropic glutamate receptor signaling pathway [ISO, ISS]
- locomotory behavior [ISO, ISS]
- mRNA polyadenylation [ISO, ISS]
- mating behavior [ISO, ISS]
- negative regulation of endopeptidase activity [ISO]
- negative regulation of neuron differentiation [ISO]
- neuromuscular process controlling balance [ISO]
- neuron apoptotic process [ISO]
- neuron projection development [ISO, ISS]
- neuron remodeling [ISO, ISS]
- positive regulation of G2/M transition of mitotic cell cycle [ISO]
- positive regulation of mitotic cell cycle [ISO, ISS]
- positive regulation of peptidase activity [IDA]
- positive regulation of transcription from RNA polymerase II promoter [ISO]
- protein phosphorylation [ISO, ISS]
- regulation of epidermal growth factor-activated receptor activity [ISO, ISS]
- regulation of gene expression [ISO]
- regulation of multicellular organism growth [ISO, ISS]
- regulation of protein binding [ISO]
- regulation of synapse structure or activity [ISO, ISS]
- regulation of translation [ISO, ISS]
- response to oxidative stress [ISO]
- smooth endoplasmic reticulum calcium ion homeostasis [ISO]
- suckling behavior [ISO]
- synaptic growth at neuromuscular junction [ISO]
- visual learning [ISO, ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- ER to Golgi transport vesicle [ISO]
- Golgi apparatus [IDA, ISO, ISS]
- apical part of cell [ISO]
- axon [IDA, ISO, ISS]
- cell [ISO]
- cell surface [IDA, ISO]
- cell-cell junction [ISO]
- ciliary rootlet [ISO]
- cytoplasm [ISO, ISS]
- cytoplasmic vesicle [ISO]
- dendritic shaft [ISO]
- dendritic spine [ISO]
- endosome [ISO]
- extracellular space [ISO]
- extracellular vesicular exosome [ISO]
- integral component of membrane [ISO, ISS]
- intracellular [ISO]
- intracellular membrane-bounded organelle [ISO]
- membrane [ISO]
- membrane raft [ISO]
- neuromuscular junction [ISO]
- neuron projection [IDA, ISO]
- nuclear envelope lumen [ISO]
- perinuclear region of cytoplasm [ISO]
- plasma membrane [ISO]
- receptor complex [ISO]
- smooth endoplasmic reticulum [ISO]
- spindle midzone [ISO]
- synapse [ISO]
- synaptic vesicle [IDA]
- terminal bouton [IDA]
Rattus norvegicus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Synaptic Protein Ubiquitination in Rat Brain Revealed by Antibody-based Ubiquitome Analysis.
Protein ubiquitination is an essential post-translational modification regulating neurodevelopment, synaptic plasticity, learning, and memory, and its dysregulation contributes to the pathogenesis of neurological diseases. Here we report a systematic analysis of ubiquitinated proteome (ubiquitome) in rat brain using a newly developed monoclonal antibody that recognizes the diglycine tag on lysine residues in trypsinized peptides (K-GG peptides). Initial antibody specificity analysis ... [more]
J. Proteome Res. Aug. 15, 2012; 0(0); [Pubmed: 22871113]
Throughput
- Low Throughput
Curated By
- BioGRID