BAIT
UBC
ubiquitin C
GO Process (2)
GO Function (2)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Rattus norvegicus
PREY
CDH2
N-cadherin
cadherin 2
GO Process (19)
GO Function (9)
GO Component (15)
Gene Ontology Biological Process
- blood vessel morphogenesis [ISO]
- calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules [IMP, ISO]
- cell adhesion [ISO]
- cell migration [ISO]
- cell-cell adhesion mediated by cadherin [ISO, ISS]
- glial cell differentiation [ISO, ISS]
- heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules [ISO]
- homophilic cell adhesion via plasma membrane adhesion molecules [ISO]
- negative regulation of canonical Wnt signaling pathway [ISO]
- neuronal stem cell maintenance [ISO, ISS]
- positive regulation of MAPK cascade [ISO]
- protein heterooligomerization [IPI]
- regulation of Rho protein signal transduction [IMP]
- regulation of axonogenesis [IMP]
- regulation of myelination [IMP]
- regulation of protein localization [IMP]
- single organismal cell-cell adhesion [IDA]
- striated muscle cell differentiation [ISO]
- synapse assembly [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- T-tubule [IDA]
- adherens junction [ISO]
- apical plasma membrane [ISO]
- catenin complex [ISO]
- cell-cell adherens junction [IDA, ISO]
- cell-cell junction [ISO]
- extracellular vesicular exosome [ISO]
- fascia adherens [IDA, ISO]
- focal adhesion [ISO]
- intercalated disc [IDA, ISO]
- lamellipodium [ISO]
- membrane [IDA]
- plasma membrane [IDA]
- protein complex [IDA]
- synapse [IDA, ISO]
Rattus norvegicus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Synaptic Protein Ubiquitination in Rat Brain Revealed by Antibody-based Ubiquitome Analysis.
Protein ubiquitination is an essential post-translational modification regulating neurodevelopment, synaptic plasticity, learning, and memory, and its dysregulation contributes to the pathogenesis of neurological diseases. Here we report a systematic analysis of ubiquitinated proteome (ubiquitome) in rat brain using a newly developed monoclonal antibody that recognizes the diglycine tag on lysine residues in trypsinized peptides (K-GG peptides). Initial antibody specificity analysis ... [more]
J. Proteome Res. Aug. 15, 2012; 0(0); [Pubmed: 22871113]
Throughput
- Low Throughput
Curated By
- BioGRID