BAIT
UBC
ubiquitin C
GO Process (2)
GO Function (2)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Rattus norvegicus
PREY
CAMK2D
CAMK1, Camk2, Camki, RATCAMKI
calcium/calmodulin-dependent protein kinase II delta
GO Process (33)
GO Function (9)
GO Component (13)
Gene Ontology Biological Process
- G1/S transition of mitotic cell cycle [ISO]
- calcium ion transport [ISO]
- cardiac muscle cell contraction [IDA]
- cardiac muscle contraction [ISO]
- cell growth involved in cardiac muscle cell development [IMP]
- cellular potassium ion homeostasis [IMP]
- endoplasmic reticulum calcium ion homeostasis [IDA]
- negative regulation of sodium ion transmembrane transport [ISO]
- negative regulation of sodium ion transmembrane transporter activity [ISO]
- peptidyl-serine phosphorylation [IDA, ISO]
- peptidyl-threonine phosphorylation [IDA, ISO]
- positive regulation of ERK1 and ERK2 cascade [IMP]
- positive regulation of Rac protein signal transduction [IMP]
- positive regulation of cardiac muscle hypertrophy [ISO, ISS]
- positive regulation of smooth muscle cell migration [IMP]
- positive regulation of smooth muscle cell proliferation [IMP]
- protein autophosphorylation [IDA, ISO]
- protein oligomerization [ISO]
- protein phosphorylation [IDA, ISO, ISS]
- regulation of G2/M transition of mitotic cell cycle [IMP]
- regulation of cardiac muscle cell action potential [IDA]
- regulation of cardiac muscle cell action potential involved in regulation of contraction [IC]
- regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion [IC]
- regulation of cell communication by electrical coupling [IDA]
- regulation of cell communication by electrical coupling involved in cardiac conduction [IC]
- regulation of cellular localization [ISO, ISS]
- regulation of generation of L-type calcium current [IDA]
- regulation of membrane depolarization [ISO]
- regulation of relaxation of cardiac muscle [IDA, ISO]
- regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum [IDA]
- regulation of sodium ion transport [ISO]
- relaxation of cardiac muscle [IDA]
- response to hypoxia [IMP]
Gene Ontology Molecular Function- calmodulin binding [ISO]
- calmodulin-dependent protein kinase activity [IDA, IMP, ISO]
- ion channel binding [IDA, IPI, ISO]
- nitric-oxide synthase binding [IPI]
- protein binding [IPI]
- protein homodimerization activity [ISO]
- protein serine/threonine kinase activity [ISO]
- sodium channel inhibitor activity [ISO]
- titin binding [ISO]
- calmodulin binding [ISO]
- calmodulin-dependent protein kinase activity [IDA, IMP, ISO]
- ion channel binding [IDA, IPI, ISO]
- nitric-oxide synthase binding [IPI]
- protein binding [IPI]
- protein homodimerization activity [ISO]
- protein serine/threonine kinase activity [ISO]
- sodium channel inhibitor activity [ISO]
- titin binding [ISO]
Gene Ontology Cellular Component
- T-tubule [ISO]
- axon initial segment [ISO]
- calcium channel complex [IDA, ISO]
- cytoplasm [IDA, ISO]
- cytosol [IDA]
- intercalated disc [ISO]
- membrane [ISO]
- neuromuscular junction [ISO]
- neuronal cell body [ISO]
- nucleus [IDA, ISO]
- perinuclear region of cytoplasm [IDA]
- protein complex [IDA]
- sarcoplasmic reticulum [ISO]
Rattus norvegicus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Synaptic Protein Ubiquitination in Rat Brain Revealed by Antibody-based Ubiquitome Analysis.
Protein ubiquitination is an essential post-translational modification regulating neurodevelopment, synaptic plasticity, learning, and memory, and its dysregulation contributes to the pathogenesis of neurological diseases. Here we report a systematic analysis of ubiquitinated proteome (ubiquitome) in rat brain using a newly developed monoclonal antibody that recognizes the diglycine tag on lysine residues in trypsinized peptides (K-GG peptides). Initial antibody specificity analysis ... [more]
J. Proteome Res. Aug. 15, 2012; 0(0); [Pubmed: 22871113]
Throughput
- Low Throughput
Curated By
- BioGRID