BAIT
UBC
ubiquitin C
GO Process (2)
GO Function (2)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Rattus norvegicus
PREY
CLIC4
chloride intracellular channel 4
GO Process (12)
GO Function (3)
GO Component (20)
Gene Ontology Biological Process
- angiogenesis [ISO]
- branching morphogenesis of an epithelial tube [ISO]
- cellular response to calcium ion [ISO]
- chloride transmembrane transport [IDA]
- chloride transport [TAS]
- endothelial cell morphogenesis [ISO]
- fertilization [ISO]
- keratinocyte differentiation [ISO]
- multicellular organism growth [ISO]
- negative regulation of cell migration [ISO, ISS]
- retina vasculature morphogenesis in camera-type eye [ISO]
- vacuolar acidification [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- apical part of cell [ISO]
- cell [ISO]
- cell surface [ISO]
- cell-cell junction [ISO]
- centrosome [ISO]
- cytoplasm [ISO, ISS, TAS]
- cytosol [ISO]
- endoplasmic reticulum [TAS]
- extracellular vesicular exosome [ISO]
- integral component of membrane [TAS]
- intracellular [ISO]
- microtubule cytoskeleton [ISO]
- microvillus [ISO]
- midbody [ISO]
- mitochondrion [ISO]
- nuclear matrix [ISO]
- perinuclear region of cytoplasm [ISO]
- plasma membrane [ISO]
- vacuole [ISO]
- vesicle [TAS]
Rattus norvegicus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Synaptic Protein Ubiquitination in Rat Brain Revealed by Antibody-based Ubiquitome Analysis.
Protein ubiquitination is an essential post-translational modification regulating neurodevelopment, synaptic plasticity, learning, and memory, and its dysregulation contributes to the pathogenesis of neurological diseases. Here we report a systematic analysis of ubiquitinated proteome (ubiquitome) in rat brain using a newly developed monoclonal antibody that recognizes the diglycine tag on lysine residues in trypsinized peptides (K-GG peptides). Initial antibody specificity analysis ... [more]
J. Proteome Res. Aug. 15, 2012; 0(0); [Pubmed: 22871113]
Throughput
- Low Throughput
Curated By
- BioGRID