BAIT
UBC
ubiquitin C
GO Process (2)
GO Function (2)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Rattus norvegicus
PREY
CFL1
cofilin 1, non-muscle
GO Process (16)
GO Function (2)
GO Component (10)
Gene Ontology Biological Process
- actin filament organization [ISO]
- cell projection organization [IDA]
- cellular component movement [ISO]
- cytoskeleton organization [ISO, ISS]
- establishment of cell polarity [ISO]
- mitotic cytokinesis [ISO]
- negative regulation of cell size [IDA]
- neural crest cell migration [ISO]
- neural fold formation [ISO]
- positive regulation of actin filament depolymerization [ISO]
- protein import into nucleus [IMP]
- protein phosphorylation [ISO]
- regulation of cell morphogenesis [ISO, ISS]
- regulation of dendritic spine morphogenesis [ISO, ISS]
- response to amino acid [ISO]
- response to virus [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Rattus norvegicus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Synaptic Protein Ubiquitination in Rat Brain Revealed by Antibody-based Ubiquitome Analysis.
Protein ubiquitination is an essential post-translational modification regulating neurodevelopment, synaptic plasticity, learning, and memory, and its dysregulation contributes to the pathogenesis of neurological diseases. Here we report a systematic analysis of ubiquitinated proteome (ubiquitome) in rat brain using a newly developed monoclonal antibody that recognizes the diglycine tag on lysine residues in trypsinized peptides (K-GG peptides). Initial antibody specificity analysis ... [more]
J. Proteome Res. Aug. 15, 2012; 0(0); [Pubmed: 22871113]
Throughput
- Low Throughput
Curated By
- BioGRID