BAIT
UBC
ubiquitin C
GO Process (2)
GO Function (2)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Rattus norvegicus
PREY
CUL3
cullin 3
GO Process (21)
GO Function (6)
GO Component (7)
Gene Ontology Biological Process
- COPII vesicle coating [ISO, ISS]
- ER to Golgi vesicle-mediated transport [ISO, ISS]
- Wnt signaling pathway [ISO]
- cell migration [ISO]
- cell morphogenesis [ISO]
- embryonic cleavage [ISO, ISS]
- gastrulation [ISO]
- in utero embryonic development [ISO]
- integrin-mediated signaling pathway [ISO, ISS]
- mitotic metaphase plate congression [ISO]
- negative regulation of Rho protein signal transduction [ISO]
- negative regulation of cyclin-dependent protein serine/threonine kinase by cyclin degradation [ISO]
- positive regulation of cytokinesis [ISO, ISS]
- positive regulation of mitotic metaphase/anaphase transition [ISO]
- proteasome-mediated ubiquitin-dependent protein catabolic process [ISO, ISS]
- protein monoubiquitination [ISO, ISS]
- protein polyubiquitination [ISO, ISS]
- protein ubiquitination [ISO]
- stem cell division [ISO, ISS]
- stress fiber assembly [ISO]
- trophectodermal cellular morphogenesis [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Rattus norvegicus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Synaptic Protein Ubiquitination in Rat Brain Revealed by Antibody-based Ubiquitome Analysis.
Protein ubiquitination is an essential post-translational modification regulating neurodevelopment, synaptic plasticity, learning, and memory, and its dysregulation contributes to the pathogenesis of neurological diseases. Here we report a systematic analysis of ubiquitinated proteome (ubiquitome) in rat brain using a newly developed monoclonal antibody that recognizes the diglycine tag on lysine residues in trypsinized peptides (K-GG peptides). Initial antibody specificity analysis ... [more]
J. Proteome Res. Aug. 15, 2012; 0(0); [Pubmed: 22871113]
Throughput
- Low Throughput
Curated By
- BioGRID