BAIT
UBC
ubiquitin C
GO Process (2)
GO Function (2)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Rattus norvegicus
PREY
MAP2K2
Prkmk2
mitogen activated protein kinase kinase 2
GO Process (9)
GO Function (8)
GO Component (15)
Gene Ontology Biological Process
- MAPK cascade [IMP]
- activation of MAPK activity [TAS]
- peptidyl-serine autophosphorylation [ISO]
- positive regulation of cell motility [ISO]
- positive regulation of protein serine/threonine kinase activity [ISO]
- protein phosphorylation [IDA]
- regulation of Golgi inheritance [TAS]
- regulation of early endosome to late endosome transport [TAS]
- regulation of stress-activated MAPK cascade [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- Golgi apparatus [ISO, TAS]
- cell cortex [IDA]
- cell-cell junction [ISO]
- cytoplasm [ISO]
- cytoplasmic side of plasma membrane [ISO]
- cytosol [IDA, TAS]
- early endosome [TAS]
- endoplasmic reticulum [ISO]
- focal adhesion [TAS]
- late endosome [TAS]
- microtubule [ISO]
- mitochondrion [TAS]
- nucleus [TAS]
- perinuclear region of cytoplasm [ISO]
- peroxisomal membrane [ISO]
Rattus norvegicus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Synaptic Protein Ubiquitination in Rat Brain Revealed by Antibody-based Ubiquitome Analysis.
Protein ubiquitination is an essential post-translational modification regulating neurodevelopment, synaptic plasticity, learning, and memory, and its dysregulation contributes to the pathogenesis of neurological diseases. Here we report a systematic analysis of ubiquitinated proteome (ubiquitome) in rat brain using a newly developed monoclonal antibody that recognizes the diglycine tag on lysine residues in trypsinized peptides (K-GG peptides). Initial antibody specificity analysis ... [more]
J. Proteome Res. Aug. 15, 2012; 0(0); [Pubmed: 22871113]
Throughput
- Low Throughput
Curated By
- BioGRID