BAIT
UBC
ubiquitin C
GO Process (2)
GO Function (2)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Rattus norvegicus
PREY
EZR
Vil2
ezrin
GO Process (9)
GO Function (7)
GO Component (30)
Gene Ontology Biological Process
- actin filament bundle assembly [ISO, ISS]
- epithelial cell differentiation [IEP]
- establishment of endothelial barrier [ISO]
- establishment or maintenance of apical/basal cell polarity [ISO]
- filopodium assembly [IMP]
- leukocyte cell-cell adhesion [ISO]
- membrane to membrane docking [ISO]
- positive regulation of gene expression [ISO]
- receptor internalization [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- Schwann cell microvillus [IDA]
- T-tubule [IDA]
- actin cytoskeleton [ISO, ISS]
- actin filament [ISO, ISS]
- apical part of cell [ISO]
- apical plasma membrane [IDA, ISO]
- astrocyte projection [IDA]
- basolateral plasma membrane [IDA]
- cell body [IDA]
- cell periphery [ISO]
- cell tip [IDA]
- ciliary basal body [ISO]
- cytosol [ISO, TAS]
- extracellular space [ISO]
- extracellular vesicular exosome [ISO]
- extrinsic component of membrane [ISO, ISS]
- filopodium [IDA, ISO]
- focal adhesion [ISO]
- intracellular [ISO]
- membrane [ISO]
- membrane raft [IDA]
- microspike [IDA]
- microvillus [ISO]
- microvillus membrane [IDA]
- myelin sheath [ISO]
- nucleolus [ISO]
- plasma membrane [IDA, ISO]
- ruffle [ISO]
- uropod [ISO]
- vesicle [ISO]
Rattus norvegicus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Synaptic Protein Ubiquitination in Rat Brain Revealed by Antibody-based Ubiquitome Analysis.
Protein ubiquitination is an essential post-translational modification regulating neurodevelopment, synaptic plasticity, learning, and memory, and its dysregulation contributes to the pathogenesis of neurological diseases. Here we report a systematic analysis of ubiquitinated proteome (ubiquitome) in rat brain using a newly developed monoclonal antibody that recognizes the diglycine tag on lysine residues in trypsinized peptides (K-GG peptides). Initial antibody specificity analysis ... [more]
J. Proteome Res. Aug. 15, 2012; 0(0); [Pubmed: 22871113]
Throughput
- Low Throughput
Curated By
- BioGRID