BAIT
UBC
ubiquitin C
GO Process (2)
GO Function (2)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Rattus norvegicus
PREY
GLUL
Glns
glutamate-ammonia ligase
GO Process (11)
GO Function (7)
GO Component (14)
Gene Ontology Biological Process
- ammonia assimilation cycle [IDA, TAS]
- cell proliferation [ISO]
- cellular response to starvation [ISO]
- glutamate metabolic process [IDA]
- glutamine biosynthetic process [IDA]
- neurotransmitter metabolic process [TAS]
- positive regulation of epithelial cell proliferation [IMP]
- positive regulation of insulin secretion [IMP]
- positive regulation of synaptic transmission, glutamatergic [IMP]
- protein homooligomerization [IDA]
- response to glucose [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- axon terminus [IDA]
- cell body [ISO]
- cell projection [IDA]
- cytoplasm [IDA, ISO]
- extracellular vesicular exosome [ISO]
- glial cell projection [ISO]
- intracellular membrane-bounded organelle [IDA]
- mitochondrion [ISO]
- myelin sheath [ISO]
- neuron projection [IDA]
- nucleus [ISO]
- perikaryon [IDA]
- protein complex [IDA]
- rough endoplasmic reticulum [IDA]
Rattus norvegicus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Synaptic Protein Ubiquitination in Rat Brain Revealed by Antibody-based Ubiquitome Analysis.
Protein ubiquitination is an essential post-translational modification regulating neurodevelopment, synaptic plasticity, learning, and memory, and its dysregulation contributes to the pathogenesis of neurological diseases. Here we report a systematic analysis of ubiquitinated proteome (ubiquitome) in rat brain using a newly developed monoclonal antibody that recognizes the diglycine tag on lysine residues in trypsinized peptides (K-GG peptides). Initial antibody specificity analysis ... [more]
J. Proteome Res. Aug. 15, 2012; 0(0); [Pubmed: 22871113]
Throughput
- Low Throughput
Curated By
- BioGRID