BAIT
UBC
ubiquitin C
GO Process (2)
GO Function (2)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Rattus norvegicus
PREY
GAPDH
BARS-38, Gapd
glyceraldehyde-3-phosphate dehydrogenase
GO Process (13)
GO Function (5)
GO Component (14)
Gene Ontology Biological Process
- apoptotic process [IMP]
- carbohydrate metabolic process [IDA]
- cellular response to interferon-gamma [ISO]
- gluconeogenesis [IDA]
- glycolytic process [IDA]
- microtubule cytoskeleton organization [IDA]
- multicellular organismal development [ISO]
- negative regulation of translation [ISO]
- neuron apoptotic process [IDA, IMP]
- oxidation-reduction process [ISO]
- peptidyl-cysteine S-trans-nitrosylation [IDA]
- protein stabilization [IDA]
- response to ammonium ion [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- GAIT complex [ISO, ISS]
- cytoplasm [IDA, ISO]
- cytosol [IDA, ISO]
- extracellular vesicular exosome [ISO]
- intracellular membrane-bounded organelle [ISO]
- lipid particle [ISO]
- membrane [ISO]
- microtubule cytoskeleton [IDA]
- mitochondrion [ISO]
- nuclear membrane [ISO]
- nucleus [IDA, ISO]
- plasma membrane [ISO]
- ribonucleoprotein complex [ISO]
- vesicle [ISO]
Rattus norvegicus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Synaptic Protein Ubiquitination in Rat Brain Revealed by Antibody-based Ubiquitome Analysis.
Protein ubiquitination is an essential post-translational modification regulating neurodevelopment, synaptic plasticity, learning, and memory, and its dysregulation contributes to the pathogenesis of neurological diseases. Here we report a systematic analysis of ubiquitinated proteome (ubiquitome) in rat brain using a newly developed monoclonal antibody that recognizes the diglycine tag on lysine residues in trypsinized peptides (K-GG peptides). Initial antibody specificity analysis ... [more]
J. Proteome Res. Aug. 15, 2012; 0(0); [Pubmed: 22871113]
Throughput
- Low Throughput
Curated By
- BioGRID