BAIT
UBC
ubiquitin C
GO Process (2)
GO Function (2)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Rattus norvegicus
PREY
GNAQ
Galphaq
guanine nucleotide binding protein (G protein), q polypeptide
GO Process (24)
GO Function (8)
GO Component (8)
Gene Ontology Biological Process
- G-protein coupled receptor signaling pathway [IDA, ISO]
- action potential [IBA, ISO]
- activation of phospholipase C activity [IMP]
- adenylate cyclase-activating G-protein coupled receptor signaling pathway [IBA, ISO]
- developmental pigmentation [ISO]
- embryonic digit morphogenesis [ISO]
- forebrain neuron development [ISO]
- glutamate receptor signaling pathway [IBA, ISO]
- heart development [ISO]
- maternal behavior [ISO]
- negative regulation of apoptotic process [IMP]
- negative regulation of potassium ion transport [IMP]
- negative regulation of protein kinase activity [ISO]
- neuron remodeling [ISO]
- phospholipase C-activating G-protein coupled receptor signaling pathway [IMP]
- phospholipase C-activating dopamine receptor signaling pathway [IBA, ISO]
- positive regulation of GTPase activity [IBA, ISO]
- positive regulation of apoptotic process [TAS]
- positive regulation of smooth muscle cell proliferation [IMP]
- post-embryonic development [ISO]
- protein stabilization [ISO]
- regulation of catenin import into nucleus [ISO]
- regulation of melanocyte differentiation [ISO]
- skeletal system development [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Rattus norvegicus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Synaptic Protein Ubiquitination in Rat Brain Revealed by Antibody-based Ubiquitome Analysis.
Protein ubiquitination is an essential post-translational modification regulating neurodevelopment, synaptic plasticity, learning, and memory, and its dysregulation contributes to the pathogenesis of neurological diseases. Here we report a systematic analysis of ubiquitinated proteome (ubiquitome) in rat brain using a newly developed monoclonal antibody that recognizes the diglycine tag on lysine residues in trypsinized peptides (K-GG peptides). Initial antibody specificity analysis ... [more]
J. Proteome Res. Aug. 15, 2012; 0(0); [Pubmed: 22871113]
Throughput
- Low Throughput
Curated By
- BioGRID