BAIT
UBC
ubiquitin C
GO Process (2)
GO Function (2)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Rattus norvegicus
PREY
GNB2L1
RACK1, rCG_34190
guanine nucleotide binding protein (G protein), beta polypeptide 2 like 1
GO Process (27)
GO Function (10)
GO Component (12)
Gene Ontology Biological Process
- activation of cysteine-type endopeptidase activity involved in apoptotic process [ISO]
- negative regulation of Wnt signaling pathway [ISS]
- negative regulation of cell growth [ISO, ISS]
- negative regulation of gene expression [ISO]
- negative regulation of hydrogen peroxide-induced neuron death [ISO]
- negative regulation of phagocytosis [ISO, ISS]
- negative regulation of protein kinase B signaling [ISO]
- negative regulation of protein tyrosine kinase activity [ISO]
- negative regulation of translation [IMP]
- osteoblast differentiation [IDA]
- positive regulation of GTPase activity [ISO, ISS]
- positive regulation of apoptotic process [ISO, ISS]
- positive regulation of cAMP catabolic process [ISO]
- positive regulation of cell migration [ISO, ISS]
- positive regulation of cyclic-nucleotide phosphodiesterase activity [ISO]
- positive regulation of gastrulation [ISS]
- positive regulation of intrinsic apoptotic signaling pathway [ISO]
- positive regulation of mitochondrial depolarization [ISO]
- positive regulation of proteasomal ubiquitin-dependent protein catabolic process [ISO, ISS]
- positive regulation of protein homooligomerization [ISO, ISS]
- positive regulation of protein phosphorylation [ISO, ISS]
- protein kinase C-activating G-protein coupled receptor signaling pathway [TAS]
- regulation of cell cycle [ISO, ISS]
- regulation of cell division [ISS]
- regulation of establishment of cell polarity [ISS]
- regulation of establishment of protein localization to plasma membrane [IMP]
- regulation of protein localization [ISS]
Gene Ontology Molecular Function- SH2 domain binding [ISO, ISS]
- cysteine-type endopeptidase activator activity involved in apoptotic process [ISO]
- enzyme binding [ISO]
- ion channel inhibitor activity [ISO]
- poly(A) RNA binding [ISO]
- protein homodimerization activity [ISO]
- protein kinase C binding [IC, IDA, ISO]
- protein phosphatase binding [ISO]
- protein tyrosine kinase inhibitor activity [ISO, ISS]
- receptor tyrosine kinase binding [ISO, ISS]
- SH2 domain binding [ISO, ISS]
- cysteine-type endopeptidase activator activity involved in apoptotic process [ISO]
- enzyme binding [ISO]
- ion channel inhibitor activity [ISO]
- poly(A) RNA binding [ISO]
- protein homodimerization activity [ISO]
- protein kinase C binding [IC, IDA, ISO]
- protein phosphatase binding [ISO]
- protein tyrosine kinase inhibitor activity [ISO, ISS]
- receptor tyrosine kinase binding [ISO, ISS]
Gene Ontology Cellular Component
Rattus norvegicus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Synaptic Protein Ubiquitination in Rat Brain Revealed by Antibody-based Ubiquitome Analysis.
Protein ubiquitination is an essential post-translational modification regulating neurodevelopment, synaptic plasticity, learning, and memory, and its dysregulation contributes to the pathogenesis of neurological diseases. Here we report a systematic analysis of ubiquitinated proteome (ubiquitome) in rat brain using a newly developed monoclonal antibody that recognizes the diglycine tag on lysine residues in trypsinized peptides (K-GG peptides). Initial antibody specificity analysis ... [more]
J. Proteome Res. Aug. 15, 2012; 0(0); [Pubmed: 22871113]
Throughput
- Low Throughput
Curated By
- BioGRID