BAIT
UBC
ubiquitin C
GO Process (2)
GO Function (2)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Rattus norvegicus
PREY
HMGB1
Ac2-008, Hmg1
high mobility group box 1
GO Process (47)
GO Function (22)
GO Component (7)
Gene Ontology Biological Process
- DNA geometric change [IDA]
- V(D)J recombination [ISO]
- actin cytoskeleton reorganization [IMP]
- base-excision repair, DNA ligation [ISO]
- cell morphogenesis [IMP]
- cellular response to interleukin-1 [IEP]
- chemotaxis [IDA]
- chromatin remodeling [IBA]
- circadian rhythm [IEP]
- eye development [ISO]
- induction of positive chemotaxis [IDA]
- inflammatory response to antigenic stimulus [ISO]
- lung development [ISO]
- male-specific defense response to bacterium [IDA]
- myoblast proliferation [IMP]
- negative regulation of DNA replication [IDA]
- negative regulation of RNA polymerase II transcriptional preinitiation complex assembly [ISO]
- negative regulation of transcription from RNA polymerase II promoter [ISO]
- nervous system development [IMP]
- neuron projection development [IGI]
- positive regulation of DNA binding [ISO]
- positive regulation of apoptotic process [IMP, ISO]
- positive regulation of cell death [IMP]
- positive regulation of cell migration [IDA]
- positive regulation of cell proliferation [IDA]
- positive regulation of cysteine-type endopeptidase activity involved in apoptotic process [ISO]
- positive regulation of glycogen catabolic process [ISO]
- positive regulation of mesenchymal cell proliferation [IDA]
- positive regulation of mitotic cell cycle [IDA]
- positive regulation of myeloid cell apoptotic process [IDA]
- positive regulation of myeloid cell differentiation [ISO]
- positive regulation of myoblast differentiation [IMP]
- positive regulation of neuron projection development [IMP]
- positive regulation of protein kinase activity [ISO]
- positive regulation of protein phosphorylation [ISO]
- positive regulation of smooth muscle cell migration [IMP]
- positive regulation of transcription from RNA polymerase II promoter [ISO]
- regulation of inflammatory response [IDA]
- regulation of transcription from RNA polymerase II promoter [ISO]
- regulation of transcription, DNA-templated [IBA]
- response to drug [IEP]
- response to glucocorticoid [ISO]
- response to glucose [IEP]
- response to heat [IEP]
- response to insulin [IEP]
- response to interferon-gamma [IMP]
- response to lipopolysaccharide [IEP]
Gene Ontology Molecular Function- 5S rRNA binding [IDA]
- DNA binding, bending [IDA, ISO]
- RAGE receptor binding [IMP, IPI]
- bent DNA binding [IDA]
- calcium-dependent protein kinase regulator activity [ISO]
- chromatin binding [IBA]
- crossed form four-way junction DNA binding [IDA]
- cytokine activity [IDA, ISO]
- double-stranded DNA binding [IDA]
- four-way junction DNA binding [IDA]
- glycolipid binding [IDA]
- heparin binding [IDA, TAS]
- open form four-way junction DNA binding [IDA]
- peptide binding [IDA]
- poly(A) RNA binding [ISO]
- protein binding [IPI]
- protein dimerization activity [IDA]
- protein kinase activator activity [ISO]
- repressing transcription factor binding [ISO]
- sequence-specific DNA binding transcription factor activity [ISO]
- single-stranded DNA binding [IDA]
- transcription factor binding [IDA, ISO]
- 5S rRNA binding [IDA]
- DNA binding, bending [IDA, ISO]
- RAGE receptor binding [IMP, IPI]
- bent DNA binding [IDA]
- calcium-dependent protein kinase regulator activity [ISO]
- chromatin binding [IBA]
- crossed form four-way junction DNA binding [IDA]
- cytokine activity [IDA, ISO]
- double-stranded DNA binding [IDA]
- four-way junction DNA binding [IDA]
- glycolipid binding [IDA]
- heparin binding [IDA, TAS]
- open form four-way junction DNA binding [IDA]
- peptide binding [IDA]
- poly(A) RNA binding [ISO]
- protein binding [IPI]
- protein dimerization activity [IDA]
- protein kinase activator activity [ISO]
- repressing transcription factor binding [ISO]
- sequence-specific DNA binding transcription factor activity [ISO]
- single-stranded DNA binding [IDA]
- transcription factor binding [IDA, ISO]
Gene Ontology Cellular Component
Rattus norvegicus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Synaptic Protein Ubiquitination in Rat Brain Revealed by Antibody-based Ubiquitome Analysis.
Protein ubiquitination is an essential post-translational modification regulating neurodevelopment, synaptic plasticity, learning, and memory, and its dysregulation contributes to the pathogenesis of neurological diseases. Here we report a systematic analysis of ubiquitinated proteome (ubiquitome) in rat brain using a newly developed monoclonal antibody that recognizes the diglycine tag on lysine residues in trypsinized peptides (K-GG peptides). Initial antibody specificity analysis ... [more]
J. Proteome Res. Aug. 15, 2012; 0(0); [Pubmed: 22871113]
Throughput
- Low Throughput
Curated By
- BioGRID