UBC
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
ITPR1
Gene Ontology Biological Process
- calcium ion transmembrane transport [ISO]
- calcium ion transport [ISO]
- cellular response to cAMP [IDA]
- cellular response to hypoxia [IEP]
- endoplasmic reticulum calcium ion homeostasis [ISO]
- inositol phosphate-mediated signaling [IDA, ISO, ISS]
- intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress [ISO, ISS]
- negative regulation of calcium-mediated signaling [ISO]
- negative regulation of neuron death [IMP]
- positive regulation of calcium ion transport [IDA]
- positive regulation of cytosolic calcium ion concentration [IMP]
- post-embryonic development [ISO]
- release of sequestered calcium ion into cytosol [IMP, ISO]
- response to hypoxia [ISO]
- voluntary musculoskeletal movement [ISO]
Gene Ontology Molecular Function- calcium channel inhibitor activity [ISO]
- inositol 1,4,5-trisphosphate-sensitive calcium-release channel activity [IDA, ISO, ISS]
- intracellular ligand-gated calcium channel activity [ISO, ISS]
- phosphatidylinositol binding [ISO, ISS]
- protein C-terminus binding [IPI]
- protein binding [IPI]
- protein complex binding [IDA]
- protein phosphatase binding [IDA]
- calcium channel inhibitor activity [ISO]
- inositol 1,4,5-trisphosphate-sensitive calcium-release channel activity [IDA, ISO, ISS]
- intracellular ligand-gated calcium channel activity [ISO, ISS]
- phosphatidylinositol binding [ISO, ISS]
- protein C-terminus binding [IPI]
- protein binding [IPI]
- protein complex binding [IDA]
- protein phosphatase binding [IDA]
Gene Ontology Cellular Component
- calcineurin complex [ISO]
- cell [ISO]
- cytoplasm [IDA, ISO]
- cytosol [ISO]
- dendrite [IDA]
- endoplasmic reticulum [ISO]
- endoplasmic reticulum membrane [IDA, ISO, TAS]
- intracellular [ISO]
- intracellular membrane-bounded organelle [IDA]
- membrane [ISO]
- membrane raft [IDA]
- neuronal cell body [IDA]
- nuclear envelope [IDA, ISO]
- nuclear inner membrane [ISO]
- nucleolus [ISO]
- perinuclear region of cytoplasm [IDA]
- plasma membrane [IDA]
- platelet dense granule membrane [ISO]
- platelet dense tubular network [ISO]
- postsynaptic density [IDA, ISO]
- protein complex [IDA, ISO]
- sarcoplasmic reticulum [IDA, ISO]
- secretory granule membrane [IDA]
- synaptic membrane [IDA]
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Synaptic Protein Ubiquitination in Rat Brain Revealed by Antibody-based Ubiquitome Analysis.
Protein ubiquitination is an essential post-translational modification regulating neurodevelopment, synaptic plasticity, learning, and memory, and its dysregulation contributes to the pathogenesis of neurological diseases. Here we report a systematic analysis of ubiquitinated proteome (ubiquitome) in rat brain using a newly developed monoclonal antibody that recognizes the diglycine tag on lysine residues in trypsinized peptides (K-GG peptides). Initial antibody specificity analysis ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| ITPR1 UBC | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | Low | - | BioGRID | - | |
| ITPR1 UBC | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| ITPR1 UBC | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| ITPR1 UBC | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - |
Curated By
- BioGRID