BAIT
UBC
ubiquitin C
GO Process (2)
GO Function (2)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Rattus norvegicus
PREY
SLC11A2
Dmt1, Nramp2
solute carrier family 11 (proton-coupled divalent metal ion transporter), member 2
GO Process (39)
GO Function (22)
GO Component (24)
Gene Ontology Biological Process
- activation of cysteine-type endopeptidase activity involved in apoptotic process [ISO]
- cadmium ion transmembrane transport [ISO]
- cation transmembrane transport [ISO]
- cellular copper ion homeostasis [IMP]
- cellular response to hypoxia [IEP]
- cellular response to iron ion [IEP]
- cellular response to oxidative stress [ISO]
- cellular response to tumor necrosis factor [IEP]
- cobalt ion transport [ISO]
- copper ion import into cell [IMP]
- copper ion transmembrane transport [IDA, IMP, ISO]
- copper ion transport [ISO]
- dendrite morphogenesis [ISO]
- detection of oxygen [ISO]
- erythrocyte development [ISO]
- ferrous iron import [ISO]
- ferrous iron transport [IMP, ISO]
- heme biosynthetic process [ISO]
- hydrogen ion transmembrane transport [ISO]
- iron ion transmembrane transport [IDA, ISO]
- iron ion transport [ISO]
- lead ion transport [ISO]
- learning or memory [ISO]
- manganese ion transmembrane transport [ISO]
- manganese ion transport [ISO]
- multicellular organismal iron ion homeostasis [ISO]
- nickel cation transmembrane transport [ISO]
- nickel cation transport [ISO]
- porphyrin-containing compound biosynthetic process [ISO]
- porphyrin-containing compound metabolic process [ISO]
- proton transport [ISO]
- response to cadmium ion [IEP]
- response to hypoxia [IEP, ISO]
- response to iron ion [IEP, ISO]
- response to lead ion [IEP]
- response to manganese ion [IEP, IMP]
- transition metal ion transport [IDA]
- vanadium ion transport [ISO]
- zinc ion transmembrane transport [ISO]
Gene Ontology Molecular Function- cadmium ion binding [IDA]
- cadmium ion transmembrane transporter activity [IDA, ISO]
- calcium ion transmembrane transporter activity [ISO]
- chromium ion transmembrane transporter activity [ISO]
- cobalt ion binding [IDA]
- cobalt ion transmembrane transporter activity [IDA, ISO]
- copper ion binding [IDA]
- copper ion transmembrane transporter activity [IDA, IMP, ISO]
- ferrous iron transmembrane transporter activity [IMP, ISO]
- hydrogen ion transmembrane transporter activity [ISO]
- inorganic cation transmembrane transporter activity [ISO]
- iron ion binding [IDA]
- iron ion transmembrane transporter activity [IDA, ISO]
- lead ion transmembrane transporter activity [IDA, ISO]
- manganese ion binding [IDA]
- manganese ion transmembrane transporter activity [IDA, ISO]
- nickel cation binding [IDA]
- nickel cation transmembrane transporter activity [IDA, ISO]
- solute:proton symporter activity [ISO]
- vanadium ion transmembrane transporter activity [ISO]
- zinc ion binding [IDA]
- zinc ion transmembrane transporter activity [ISO]
- cadmium ion binding [IDA]
- cadmium ion transmembrane transporter activity [IDA, ISO]
- calcium ion transmembrane transporter activity [ISO]
- chromium ion transmembrane transporter activity [ISO]
- cobalt ion binding [IDA]
- cobalt ion transmembrane transporter activity [IDA, ISO]
- copper ion binding [IDA]
- copper ion transmembrane transporter activity [IDA, IMP, ISO]
- ferrous iron transmembrane transporter activity [IMP, ISO]
- hydrogen ion transmembrane transporter activity [ISO]
- inorganic cation transmembrane transporter activity [ISO]
- iron ion binding [IDA]
- iron ion transmembrane transporter activity [IDA, ISO]
- lead ion transmembrane transporter activity [IDA, ISO]
- manganese ion binding [IDA]
- manganese ion transmembrane transporter activity [IDA, ISO]
- nickel cation binding [IDA]
- nickel cation transmembrane transporter activity [IDA, ISO]
- solute:proton symporter activity [ISO]
- vanadium ion transmembrane transporter activity [ISO]
- zinc ion binding [IDA]
- zinc ion transmembrane transporter activity [ISO]
Gene Ontology Cellular Component
- apical part of cell [IDA, ISO]
- apical plasma membrane [ISO]
- basal part of cell [ISO]
- brush border [ISO]
- cell surface [ISO]
- cytoplasm [IDA, ISO]
- cytoplasmic vesicle [ISO]
- early endosome [ISO]
- endomembrane system [ISO]
- endosome [ISO]
- integral component of membrane [IDA]
- integral component of plasma membrane [ISO]
- late endosome [ISO]
- late endosome membrane [IDA, ISO]
- lysosomal membrane [IDA, ISO]
- lysosome [ISO]
- membrane [ISO]
- nucleus [ISO]
- paraferritin complex [ISO]
- perinuclear region of cytoplasm [ISO]
- plasma membrane [ISO]
- recycling endosome [ISO]
- trans-Golgi network [ISO]
- vacuole [ISO]
Rattus norvegicus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Synaptic Protein Ubiquitination in Rat Brain Revealed by Antibody-based Ubiquitome Analysis.
Protein ubiquitination is an essential post-translational modification regulating neurodevelopment, synaptic plasticity, learning, and memory, and its dysregulation contributes to the pathogenesis of neurological diseases. Here we report a systematic analysis of ubiquitinated proteome (ubiquitome) in rat brain using a newly developed monoclonal antibody that recognizes the diglycine tag on lysine residues in trypsinized peptides (K-GG peptides). Initial antibody specificity analysis ... [more]
J. Proteome Res. Aug. 15, 2012; 0(0); [Pubmed: 22871113]
Throughput
- Low Throughput
Curated By
- BioGRID