BAIT
UBC
ubiquitin C
GO Process (2)
GO Function (2)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Rattus norvegicus
PREY
SLC9A3R1
solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3 regulator 1
GO Process (26)
GO Function (13)
GO Component (20)
Gene Ontology Biological Process
- actin cytoskeleton organization [ISO]
- adenylate cyclase-activating dopamine receptor signaling pathway [ISO]
- auditory receptor cell stereocilium organization [ISO]
- bile acid secretion [ISO, ISS]
- cAMP-mediated signaling [ISO]
- cellular phosphate ion homeostasis [ISO]
- cellular protein localization [ISO]
- glutathione transport [ISO, ISS]
- microvillus assembly [ISO]
- negative regulation of ERK1 and ERK2 cascade [ISO]
- negative regulation of cell migration [ISO]
- negative regulation of cell motility [ISO, ISS]
- negative regulation of cell proliferation [ISO]
- negative regulation of phosphatidylinositol 3-kinase signaling [ISO, ISS]
- negative regulation of platelet-derived growth factor receptor signaling pathway [ISO, ISS]
- negative regulation of protein kinase B signaling [ISO, ISS]
- negative regulation of sodium ion transport [ISO]
- negative regulation of sodium:proton antiporter activity [ISO]
- phospholipase C-activating dopamine receptor signaling pathway [ISO]
- positive regulation of intrinsic apoptotic signaling pathway [ISO]
- regulation of excretion [ISO]
- regulation of protein kinase activity [ISO, ISS]
- renal absorption [ISO, ISS]
- renal phosphate ion absorption [ISO, ISS]
- renal sodium ion transport [ISO]
- sensory perception of sound [ISO]
Gene Ontology Molecular Function- PDZ domain binding [IPI, ISO]
- beta-2 adrenergic receptor binding [ISO]
- beta-catenin binding [ISO]
- chloride channel regulator activity [ISO, ISS]
- dopamine receptor binding [ISO]
- growth factor receptor binding [ISO]
- phosphatase binding [ISO]
- protein binding [IPI]
- protein complex binding [IPI]
- protein complex scaffold [IMP]
- protein domain specific binding [IPI]
- protein self-association [ISO]
- receptor binding [ISO]
- PDZ domain binding [IPI, ISO]
- beta-2 adrenergic receptor binding [ISO]
- beta-catenin binding [ISO]
- chloride channel regulator activity [ISO, ISS]
- dopamine receptor binding [ISO]
- growth factor receptor binding [ISO]
- phosphatase binding [ISO]
- protein binding [IPI]
- protein complex binding [IPI]
- protein complex scaffold [IMP]
- protein domain specific binding [IPI]
- protein self-association [ISO]
- receptor binding [ISO]
Gene Ontology Cellular Component
- apical part of cell [ISO]
- apical plasma membrane [IDA, ISO]
- brush border membrane [ISO]
- cell [ISO]
- cell periphery [ISO]
- centrosome [ISO]
- cytoplasm [IDA, ISO]
- extracellular vesicular exosome [ISO]
- intracellular [ISO]
- intracellular membrane-bounded organelle [ISO]
- membrane [ISO, ISS]
- membrane raft [IDA]
- microvillus [ISO]
- microvillus membrane [IDA]
- nucleus [ISO]
- plasma membrane [ISO]
- sperm midpiece [ISO, ISS]
- stereocilium [ISO]
- stereocilium bundle tip [ISO]
- vesicle [ISO]
Rattus norvegicus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Synaptic Protein Ubiquitination in Rat Brain Revealed by Antibody-based Ubiquitome Analysis.
Protein ubiquitination is an essential post-translational modification regulating neurodevelopment, synaptic plasticity, learning, and memory, and its dysregulation contributes to the pathogenesis of neurological diseases. Here we report a systematic analysis of ubiquitinated proteome (ubiquitome) in rat brain using a newly developed monoclonal antibody that recognizes the diglycine tag on lysine residues in trypsinized peptides (K-GG peptides). Initial antibody specificity analysis ... [more]
J. Proteome Res. Aug. 15, 2012; 0(0); [Pubmed: 22871113]
Throughput
- Low Throughput
Curated By
- BioGRID