BAIT
UBC
ubiquitin C
GO Process (2)
GO Function (2)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Rattus norvegicus
PREY
NCKAP1
NCK-associated protein 1
GO Process (23)
GO Function (3)
GO Component (4)
Gene Ontology Biological Process
- Rac protein signal transduction [ISO]
- apical protein localization [ISO]
- basal protein localization [ISO]
- cell migration involved in gastrulation [ISO]
- embryonic body morphogenesis [ISO]
- embryonic foregut morphogenesis [ISO]
- embryonic heart tube development [ISO]
- endoderm development [ISO]
- establishment or maintenance of actin cytoskeleton polarity [ISO]
- in utero embryonic development [ISO]
- lamellipodium assembly [ISO]
- mesodermal cell migration [ISO]
- neural tube closure [ISO]
- notochord development [ISO]
- notochord morphogenesis [ISO]
- paraxial mesoderm development [ISO]
- paraxial mesoderm morphogenesis [ISO]
- positive regulation of Arp2/3 complex-mediated actin nucleation [ISO]
- positive regulation of lamellipodium assembly [ISO]
- protein stabilization [ISO]
- regulation of protein localization [ISO]
- somitogenesis [ISO]
- zygotic determination of anterior/posterior axis, embryo [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Rattus norvegicus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Synaptic Protein Ubiquitination in Rat Brain Revealed by Antibody-based Ubiquitome Analysis.
Protein ubiquitination is an essential post-translational modification regulating neurodevelopment, synaptic plasticity, learning, and memory, and its dysregulation contributes to the pathogenesis of neurological diseases. Here we report a systematic analysis of ubiquitinated proteome (ubiquitome) in rat brain using a newly developed monoclonal antibody that recognizes the diglycine tag on lysine residues in trypsinized peptides (K-GG peptides). Initial antibody specificity analysis ... [more]
J. Proteome Res. Aug. 15, 2012; 0(0); [Pubmed: 22871113]
Throughput
- Low Throughput
Curated By
- BioGRID