BAIT
UBC
ubiquitin C
GO Process (2)
GO Function (2)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Rattus norvegicus
PREY
NDFIP1
Nedd4 family interacting protein 1
GO Process (17)
GO Function (3)
GO Component (3)
Gene Ontology Biological Process
- cellular iron ion homeostasis [ISO]
- negative regulation of T cell proliferation [ISO]
- negative regulation of gene expression [ISO]
- negative regulation of inflammatory response [ISO]
- negative regulation of interleukin-4 production [ISO]
- negative regulation of isotype switching to IgE isotypes [ISO]
- negative regulation of protein transport [ISO]
- negative regulation of transporter activity [ISO]
- negative regulation of type 2 immune response [ISO]
- positive regulation of I-kappaB kinase/NF-kappaB signaling [ISO]
- positive regulation of protein catabolic process [ISO]
- positive regulation of protein ubiquitination [ISO]
- regulation of isotype switching to IgG isotypes [ISO]
- regulation of lymphocyte differentiation [ISO]
- regulation of myeloid leukocyte differentiation [ISO]
- signal transduction [ISO]
- ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Rattus norvegicus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Synaptic Protein Ubiquitination in Rat Brain Revealed by Antibody-based Ubiquitome Analysis.
Protein ubiquitination is an essential post-translational modification regulating neurodevelopment, synaptic plasticity, learning, and memory, and its dysregulation contributes to the pathogenesis of neurological diseases. Here we report a systematic analysis of ubiquitinated proteome (ubiquitome) in rat brain using a newly developed monoclonal antibody that recognizes the diglycine tag on lysine residues in trypsinized peptides (K-GG peptides). Initial antibody specificity analysis ... [more]
J. Proteome Res. Aug. 15, 2012; 0(0); [Pubmed: 22871113]
Throughput
- Low Throughput
Curated By
- BioGRID