BAIT
UBC
ubiquitin C
GO Process (2)
GO Function (2)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Rattus norvegicus
PREY
NEFL
NF-L, Nfl
neurofilament, light polypeptide
GO Process (21)
GO Function (6)
GO Component (8)
Gene Ontology Biological Process
- anterograde axon cargo transport [ISO]
- axon transport of mitochondrion [ISO]
- intermediate filament bundle assembly [ISO]
- intermediate filament organization [ISO]
- locomotion [ISO]
- microtubule cytoskeleton organization [ISO]
- negative regulation of neuron apoptotic process [ISO]
- neurofilament bundle assembly [ISO]
- neurofilament cytoskeleton organization [ISO]
- neuromuscular process controlling balance [ISO]
- neuron projection morphogenesis [ISO]
- peripheral nervous system axon regeneration [ISO]
- positive regulation of axonogenesis [ISO]
- protein polymerization [IDA]
- regulation of axon diameter [ISO]
- response to corticosterone [IEP]
- response to organic substance [IEP]
- response to organonitrogen compound [IEP]
- response to peptide hormone [IEP]
- response to toxic substance [IEP]
- retrograde axon cargo transport [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Rattus norvegicus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Synaptic Protein Ubiquitination in Rat Brain Revealed by Antibody-based Ubiquitome Analysis.
Protein ubiquitination is an essential post-translational modification regulating neurodevelopment, synaptic plasticity, learning, and memory, and its dysregulation contributes to the pathogenesis of neurological diseases. Here we report a systematic analysis of ubiquitinated proteome (ubiquitome) in rat brain using a newly developed monoclonal antibody that recognizes the diglycine tag on lysine residues in trypsinized peptides (K-GG peptides). Initial antibody specificity analysis ... [more]
J. Proteome Res. Aug. 15, 2012; 0(0); [Pubmed: 22871113]
Throughput
- Low Throughput
Curated By
- BioGRID