BAIT
UBC
ubiquitin C
GO Process (2)
GO Function (2)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Rattus norvegicus
PREY
NME1
NME/NM23 nucleoside diphosphate kinase 1
GO Process (17)
GO Function (15)
GO Component (10)
Gene Ontology Biological Process
- DNA catabolic process [ISO]
- cellular response to drug [IEP]
- cellular response to fatty acid [IEP]
- cellular response to glucose stimulus [IEP]
- hippocampus development [IEP]
- lactation [ISO]
- mammary gland development [ISO]
- negative regulation of gene expression [IDA]
- negative regulation of myeloid leukocyte differentiation [IDA]
- nucleoside diphosphate phosphorylation [IDA]
- positive regulation of DNA binding [ISO]
- positive regulation of epithelial cell proliferation [ISO]
- positive regulation of neuron projection development [IMP]
- response to amine [IEP]
- response to cAMP [IEP]
- response to drug [IEP]
- response to testosterone [IEP]
Gene Ontology Molecular Function- ATP binding [ISO]
- GTP binding [ISO]
- RNA polymerase II regulatory region sequence-specific DNA binding [IDA]
- deoxyribonuclease activity [ISO]
- enzyme binding [IPI]
- gamma-tubulin binding [IDA]
- identical protein binding [ISO]
- intermediate filament binding [IPI]
- magnesium ion binding [ISO]
- nucleoside diphosphate kinase activity [IDA, ISO]
- poly(A) RNA binding [ISO]
- protein binding [IPI]
- protein kinase binding [IPI]
- ribosomal small subunit binding [ISO]
- single-stranded DNA binding [IDA]
- ATP binding [ISO]
- GTP binding [ISO]
- RNA polymerase II regulatory region sequence-specific DNA binding [IDA]
- deoxyribonuclease activity [ISO]
- enzyme binding [IPI]
- gamma-tubulin binding [IDA]
- identical protein binding [ISO]
- intermediate filament binding [IPI]
- magnesium ion binding [ISO]
- nucleoside diphosphate kinase activity [IDA, ISO]
- poly(A) RNA binding [ISO]
- protein binding [IPI]
- protein kinase binding [IPI]
- ribosomal small subunit binding [ISO]
- single-stranded DNA binding [IDA]
Gene Ontology Cellular Component
Rattus norvegicus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Synaptic Protein Ubiquitination in Rat Brain Revealed by Antibody-based Ubiquitome Analysis.
Protein ubiquitination is an essential post-translational modification regulating neurodevelopment, synaptic plasticity, learning, and memory, and its dysregulation contributes to the pathogenesis of neurological diseases. Here we report a systematic analysis of ubiquitinated proteome (ubiquitome) in rat brain using a newly developed monoclonal antibody that recognizes the diglycine tag on lysine residues in trypsinized peptides (K-GG peptides). Initial antibody specificity analysis ... [more]
J. Proteome Res. Aug. 15, 2012; 0(0); [Pubmed: 22871113]
Throughput
- Low Throughput
Curated By
- BioGRID