BAIT
UBC
ubiquitin C
GO Process (2)
GO Function (2)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Rattus norvegicus
PREY
PAM
peptidylglycine alpha-amidating monooxygenase
GO Process (23)
GO Function (7)
GO Component (12)
Gene Ontology Biological Process
- central nervous system development [IEP]
- heart development [IEP]
- lactation [IEP]
- limb development [IEP]
- long-chain fatty acid metabolic process [IDA]
- maternal process involved in female pregnancy [IEP]
- odontogenesis [IEP]
- ovulation cycle process [IEP]
- peptide amidation [IDA, ISO]
- peptide metabolic process [IDA]
- protein amidation [IDA]
- protein homooligomerization [IDA]
- protein metabolic process [IDA]
- regulation of actin cytoskeleton organization [IMP]
- regulation of protein secretion [IMP]
- regulation of transcription from RNA polymerase II promoter [IMP]
- response to copper ion [IEP]
- response to drug [IDA]
- response to estradiol [IEP]
- response to glucocorticoid [IEP]
- response to hypoxia [IEP]
- response to pH [IDA]
- toxin metabolic process [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Rattus norvegicus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Synaptic Protein Ubiquitination in Rat Brain Revealed by Antibody-based Ubiquitome Analysis.
Protein ubiquitination is an essential post-translational modification regulating neurodevelopment, synaptic plasticity, learning, and memory, and its dysregulation contributes to the pathogenesis of neurological diseases. Here we report a systematic analysis of ubiquitinated proteome (ubiquitome) in rat brain using a newly developed monoclonal antibody that recognizes the diglycine tag on lysine residues in trypsinized peptides (K-GG peptides). Initial antibody specificity analysis ... [more]
J. Proteome Res. Aug. 15, 2012; 0(0); [Pubmed: 22871113]
Throughput
- Low Throughput
Curated By
- BioGRID