BAIT
UBC
ubiquitin C
GO Process (2)
GO Function (2)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Rattus norvegicus
PREY
FKBP1A
FKBP12, Fkbp2
FK506 binding protein 1a
GO Process (26)
GO Function (14)
GO Component (11)
Gene Ontology Biological Process
- SMAD protein complex assembly [ISO]
- T cell proliferation [ISO]
- amyloid fibril formation [ISO]
- chaperone-mediated protein folding [IBA]
- cytokine-mediated signaling pathway [ISO]
- extracellular fibril organization [ISO]
- heart morphogenesis [ISO]
- heart trabecula formation [ISO]
- muscle contraction [ISO]
- negative regulation of protein phosphatase type 2B activity [ISO]
- negative regulation of protein phosphorylation [ISO]
- negative regulation of release of sequestered calcium ion into cytosol [IBA, ISO]
- negative regulation of ryanodine-sensitive calcium-release channel activity [ISO]
- positive regulation of I-kappaB kinase/NF-kappaB signaling [ISO]
- positive regulation of protein binding [ISO]
- positive regulation of protein ubiquitination [ISO]
- protein peptidyl-prolyl isomerization [IBA, ISO]
- regulation of activin receptor signaling pathway [ISO]
- regulation of amyloid precursor protein catabolic process [ISO]
- regulation of immune response [ISO]
- regulation of protein localization [ISO]
- regulation of ryanodine-sensitive calcium-release channel activity [ISO]
- release of sequestered calcium ion into cytosol [ISO]
- response to caffeine [ISO]
- response to iron ion [IEP]
- ventricular cardiac muscle tissue morphogenesis [ISO]
Gene Ontology Molecular Function- FK506 binding [IDA, ISO]
- Hsp70 protein binding [IPI]
- SMAD binding [ISO]
- activin binding [ISO]
- calcium channel inhibitor activity [IBA, ISO]
- drug binding [ISO]
- enzyme binding [IPI]
- ion channel binding [ISO]
- peptidyl-prolyl cis-trans isomerase activity [IBA, ISO]
- protein binding [IPI]
- protein homodimerization activity [ISO]
- signal transducer activity [ISO]
- transforming growth factor beta receptor binding [IDA]
- type I transforming growth factor beta receptor binding [IDA]
- FK506 binding [IDA, ISO]
- Hsp70 protein binding [IPI]
- SMAD binding [ISO]
- activin binding [ISO]
- calcium channel inhibitor activity [IBA, ISO]
- drug binding [ISO]
- enzyme binding [IPI]
- ion channel binding [ISO]
- peptidyl-prolyl cis-trans isomerase activity [IBA, ISO]
- protein binding [IPI]
- protein homodimerization activity [ISO]
- signal transducer activity [ISO]
- transforming growth factor beta receptor binding [IDA]
- type I transforming growth factor beta receptor binding [IDA]
Gene Ontology Cellular Component
Rattus norvegicus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Synaptic Protein Ubiquitination in Rat Brain Revealed by Antibody-based Ubiquitome Analysis.
Protein ubiquitination is an essential post-translational modification regulating neurodevelopment, synaptic plasticity, learning, and memory, and its dysregulation contributes to the pathogenesis of neurological diseases. Here we report a systematic analysis of ubiquitinated proteome (ubiquitome) in rat brain using a newly developed monoclonal antibody that recognizes the diglycine tag on lysine residues in trypsinized peptides (K-GG peptides). Initial antibody specificity analysis ... [more]
J. Proteome Res. Aug. 15, 2012; 0(0); [Pubmed: 22871113]
Throughput
- Low Throughput
Curated By
- BioGRID