BAIT
UBC
ubiquitin C
GO Process (2)
GO Function (2)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Rattus norvegicus
PREY
PAFAH1B1
LIS1
platelet-activating factor acetylhydrolase 1b, regulatory subunit 1
GO Process (36)
GO Function (8)
GO Component (22)
Gene Ontology Biological Process
- acrosome assembly [ISO]
- actin cytoskeleton organization [ISO]
- adult locomotory behavior [ISO]
- ameboidal-type cell migration [ISO]
- brain development [IEP]
- brain morphogenesis [ISO]
- cell migration [ISO]
- cerebral cortex development [ISO]
- cerebral cortex neuron differentiation [IMP]
- corpus callosum morphogenesis [ISO]
- establishment of centrosome localization [IMP]
- establishment of mitotic spindle orientation [ISO]
- hippocampus development [ISO]
- layer formation in cerebral cortex [ISO]
- learning or memory [ISO]
- microtubule cytoskeleton organization [ISO]
- microtubule organizing center organization [ISO]
- microtubule-based process [ISO]
- negative regulation of JNK cascade [ISO]
- negative regulation of neuron projection development [IMP]
- neuroblast proliferation [ISO]
- neuromuscular process controlling balance [ISO]
- neuron migration [IMP, ISO]
- nuclear envelope disassembly [ISO]
- nuclear migration [IMP]
- osteoclast development [ISO]
- positive regulation of axon extension [IMP]
- positive regulation of cytokine-mediated signaling pathway [ISO]
- positive regulation of mitotic cell cycle [IMP]
- protein secretion [ISO]
- regulation of Rho GTPase activity [ISO]
- retrograde axon cargo transport [ISO]
- stem cell division [IMP]
- synaptic transmission [ISO]
- transmission of nerve impulse [ISO]
- vesicle transport along microtubule [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- astral microtubule [ISO]
- axon [IDA]
- cell [ISO]
- cell cortex [ISO]
- cell leading edge [ISO]
- centrosome [ISO]
- cytoplasm [ISO]
- cytosol [IDA]
- extracellular vesicular exosome [ISO]
- growth cone [IDA]
- intracellular [ISO]
- kinesin complex [IDA]
- kinetochore [ISO]
- microtubule associated complex [ISO]
- microtubule cytoskeleton [ISO]
- motile primary cilium [ISO]
- neuron projection [IDA]
- neuronal cell body [IDA]
- nonmotile primary cilium [ISO]
- nuclear envelope [IDA, ISO]
- perinuclear region of cytoplasm [ISO]
- vesicle [IDA]
Rattus norvegicus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Synaptic Protein Ubiquitination in Rat Brain Revealed by Antibody-based Ubiquitome Analysis.
Protein ubiquitination is an essential post-translational modification regulating neurodevelopment, synaptic plasticity, learning, and memory, and its dysregulation contributes to the pathogenesis of neurological diseases. Here we report a systematic analysis of ubiquitinated proteome (ubiquitome) in rat brain using a newly developed monoclonal antibody that recognizes the diglycine tag on lysine residues in trypsinized peptides (K-GG peptides). Initial antibody specificity analysis ... [more]
J. Proteome Res. Aug. 15, 2012; 0(0); [Pubmed: 22871113]
Throughput
- Low Throughput
Curated By
- BioGRID