BAIT
UBC
ubiquitin C
GO Process (2)
GO Function (2)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Rattus norvegicus
PREY
LAMTOR1
p18
late endosomal/lysosomal adaptor, MAPK and MTOR activator 1
GO Process (15)
GO Function (3)
GO Component (8)
Gene Ontology Biological Process
- cell growth [ISO, ISS]
- cellular protein localization [ISO, ISS]
- cellular response to amino acid stimulus [ISO, ISS]
- cholesterol homeostasis [ISO, ISS]
- endosome localization [ISO, ISS]
- endosome organization [ISO, ISS]
- lysosome localization [ISO, ISS]
- lysosome organization [ISO, ISS]
- positive regulation of GTPase activity [ISO]
- positive regulation of MAPK cascade [ISO, ISS]
- positive regulation of TOR signaling [ISO, ISS]
- regulation of cholesterol efflux [ISO, ISS]
- regulation of cholesterol esterification [ISO, ISS]
- regulation of cholesterol import [ISO, ISS]
- regulation of receptor recycling [ISO, ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Rattus norvegicus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Synaptic Protein Ubiquitination in Rat Brain Revealed by Antibody-based Ubiquitome Analysis.
Protein ubiquitination is an essential post-translational modification regulating neurodevelopment, synaptic plasticity, learning, and memory, and its dysregulation contributes to the pathogenesis of neurological diseases. Here we report a systematic analysis of ubiquitinated proteome (ubiquitome) in rat brain using a newly developed monoclonal antibody that recognizes the diglycine tag on lysine residues in trypsinized peptides (K-GG peptides). Initial antibody specificity analysis ... [more]
J. Proteome Res. Aug. 15, 2012; 0(0); [Pubmed: 22871113]
Throughput
- Low Throughput
Curated By
- BioGRID