BAIT
UBC
ubiquitin C
GO Process (2)
GO Function (2)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Rattus norvegicus
PREY
RAB14
RAB14, member RAS oncogene family
GO Process (12)
GO Function (6)
GO Component (23)
Gene Ontology Biological Process
- GTP catabolic process [IBA, ISO]
- Golgi to endosome transport [ISO, ISS, TAS]
- Rab protein signal transduction [IBA]
- apical protein localization [IDA]
- body fluid secretion [IMP]
- endocytic recycling [ISO, ISS]
- fibroblast growth factor receptor signaling pathway [ISO, ISS]
- intracellular protein transport [IBA]
- intracellular transport [NAS]
- phagolysosome assembly involved in apoptotic cell clearance [IBA]
- regulation of protein localization [IDA, IMP, ISO]
- vesicle-mediated transport [NAS]
Gene Ontology Molecular Function- GDP binding [IBA, ISO, ISS]
- GTP binding [IBA, ISO, ISS]
- GTPase activity [IBA, ISO, ISS, NAS]
- glycoprotein binding [IDA]
- myosin V binding [ISO]
- protein binding [IPI]
- GDP binding [IBA, ISO, ISS]
- GTP binding [IBA, ISO, ISS]
- GTPase activity [IBA, ISO, ISS, NAS]
- glycoprotein binding [IDA]
- myosin V binding [ISO]
- protein binding [IPI]
Gene Ontology Cellular Component
- Golgi stack [IDA]
- alveolar lamellar body [IDA]
- apical plasma membrane [IDA]
- cell [ISO]
- cytoplasm [ISO]
- cytoplasmic vesicle membrane [TAS]
- cytosol [IDA]
- early endosome [IDA]
- extracellular vesicular exosome [ISO]
- intracellular [ISO]
- intracellular membrane-bounded organelle [ISO]
- late endosome [IDA]
- lysosomal membrane [ISO]
- lysosome [IDA]
- nuclear outer membrane-endoplasmic reticulum membrane network [IDA]
- perinuclear region of cytoplasm [IDA]
- phagocytic vesicle [IBA, ISO, ISS]
- plasma membrane [IDA]
- primary cilium [ISO]
- recycling endosome [ISO, ISS]
- rough endoplasmic reticulum [IDA]
- trans-Golgi network transport vesicle [IDA]
- transport vesicle [IDA]
Rattus norvegicus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Synaptic Protein Ubiquitination in Rat Brain Revealed by Antibody-based Ubiquitome Analysis.
Protein ubiquitination is an essential post-translational modification regulating neurodevelopment, synaptic plasticity, learning, and memory, and its dysregulation contributes to the pathogenesis of neurological diseases. Here we report a systematic analysis of ubiquitinated proteome (ubiquitome) in rat brain using a newly developed monoclonal antibody that recognizes the diglycine tag on lysine residues in trypsinized peptides (K-GG peptides). Initial antibody specificity analysis ... [more]
J. Proteome Res. Aug. 15, 2012; 0(0); [Pubmed: 22871113]
Throughput
- Low Throughput
Curated By
- BioGRID