BAIT
UBC
ubiquitin C
GO Process (2)
GO Function (2)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Rattus norvegicus
PREY
RAB8B
RAB8B, member RAS oncogene family
GO Process (16)
GO Function (6)
GO Component (12)
Gene Ontology Biological Process
- GTP catabolic process [IBA]
- Golgi vesicle fusion to target membrane [IBA]
- Rab protein signal transduction [IBA]
- adherens junction organization [IDA]
- antigen processing and presentation [ISO]
- cellular response to insulin stimulus [IBA]
- cilium assembly [ISO]
- intracellular protein transport [IBA]
- positive regulation of cell projection organization [IDA]
- positive regulation of corticotropin secretion [IDA]
- protein import into peroxisome membrane [ISO]
- protein localization to plasma membrane [IBA]
- protein secretion [IBA]
- regulation of exocytosis [IBA]
- synaptic vesicle exocytosis [IBA]
- vesicle docking involved in exocytosis [IBA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- cell tip [IDA]
- cytoplasmic vesicle [IDA]
- endosome [IBA]
- extracellular vesicular exosome [ISO]
- mitochondrion [ISO]
- perinuclear region of cytoplasm [IDA]
- peroxisomal membrane [ISO]
- phagocytic vesicle [ISO, ISS]
- plasma membrane [IDA]
- secretory granule membrane [IBA]
- synaptic vesicle [IBA]
- trans-Golgi network transport vesicle [IBA]
Rattus norvegicus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Synaptic Protein Ubiquitination in Rat Brain Revealed by Antibody-based Ubiquitome Analysis.
Protein ubiquitination is an essential post-translational modification regulating neurodevelopment, synaptic plasticity, learning, and memory, and its dysregulation contributes to the pathogenesis of neurological diseases. Here we report a systematic analysis of ubiquitinated proteome (ubiquitome) in rat brain using a newly developed monoclonal antibody that recognizes the diglycine tag on lysine residues in trypsinized peptides (K-GG peptides). Initial antibody specificity analysis ... [more]
J. Proteome Res. Aug. 15, 2012; 0(0); [Pubmed: 22871113]
Throughput
- Low Throughput
Curated By
- BioGRID