BAIT
UBC
ubiquitin C
GO Process (2)
GO Function (2)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Rattus norvegicus
PREY
SEMA5A
sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A
GO Process (20)
GO Function (5)
GO Component (3)
Gene Ontology Biological Process
- axon guidance [ISO]
- axonal fasciculation [IDA]
- blood vessel endothelial cell proliferation involved in sprouting angiogenesis [ISO, ISS]
- branching morphogenesis of an epithelial tube [ISO]
- cell chemotaxis [ISO, ISS]
- diencephalon development [IMP]
- negative regulation of axon extension involved in axon guidance [IMP]
- negative regulation of cell adhesion [ISO, ISS]
- negative regulation of endothelial cell apoptotic process [ISO, ISS]
- patterning of blood vessels [ISO]
- positive chemotaxis [ISO, ISS]
- positive regulation of actin filament depolymerization [ISO, ISS]
- positive regulation of angiogenesis [ISO, ISS]
- positive regulation of axon extension involved in axon guidance [IMP]
- positive regulation of catenin import into nucleus [ISO, ISS]
- positive regulation of endothelial cell chemotaxis [ISO, ISS]
- positive regulation of endothelial cell proliferation [ISO, ISS]
- positive regulation of protein kinase B signaling [ISO, ISS]
- semaphorin-plexin signaling pathway [ISO, ISS]
- signal clustering [IMP, ISO, ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Rattus norvegicus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Synaptic Protein Ubiquitination in Rat Brain Revealed by Antibody-based Ubiquitome Analysis.
Protein ubiquitination is an essential post-translational modification regulating neurodevelopment, synaptic plasticity, learning, and memory, and its dysregulation contributes to the pathogenesis of neurological diseases. Here we report a systematic analysis of ubiquitinated proteome (ubiquitome) in rat brain using a newly developed monoclonal antibody that recognizes the diglycine tag on lysine residues in trypsinized peptides (K-GG peptides). Initial antibody specificity analysis ... [more]
J. Proteome Res. Aug. 15, 2012; 0(0); [Pubmed: 22871113]
Throughput
- Low Throughput
Curated By
- BioGRID